Document Detail


In vivo identification of regulators of cell invasion across basement membranes.
MedLine Citation:
PMID:  20442418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell invasion through basement membranes during development, immune surveillance, and metastasis remains poorly understood. To gain further insight into this key cellular behavior, we performed an in vivo screen for regulators of cell invasion through basement membranes, using the simple model of Caenorhabditis elegans anchor cell invasion, and identified 99 genes that promote invasion, including the genes encoding the chaperonin complex cct. Notably, most of these genes have not been previously implicated in invasive cell behavior. We characterized members of the cct complex and 11 other gene products, determining the distinct aspects of the invasive cascade that they regulate, including formation of a specialized invasive cell membrane and its ability to breach the basement membrane. RNA interference-mediated knockdown of the human orthologs of cct-5 and lit-1, which had not previously been implicated in cell invasion, reduced the invasiveness of metastatic carcinoma cells, suggesting that a conserved genetic program underlies cell invasion. These results increase our understanding of the genetic underpinnings of cell invasion and also provide new potential therapeutic targets to limit this behavior.
Authors:
David Q Matus; Xiao-Yan Li; Sarah Durbin; Daniel Agarwal; Qiuyi Chi; Stephen J Weiss; David R Sherwood
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-05-04
Journal Detail:
Title:  Science signaling     Volume:  3     ISSN:  1937-9145     ISO Abbreviation:  Sci Signal     Publication Date:  2010  
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-07-26     Revised Date:  2014-02-28    
Medline Journal Info:
Nlm Unique ID:  101465400     Medline TA:  Sci Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  ra35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Basement Membrane / cytology*
Caenorhabditis elegans / cytology*,  genetics
Neoplasm Invasiveness* / genetics
RNA Interference
Grant Support
ID/Acronym/Agency:
CA088308/CA/NCI NIH HHS; CA116516/CA/NCI NIH HHS; GM079320/GM/NIGMS NIH HHS; K01 CA098316-01/CA/NCI NIH HHS; R01 GM079320/GM/NIGMS NIH HHS
Comments/Corrections
Comment In:
Nat Rev Cancer. 2010 Jul;10(7):452   [PMID:  20589968 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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