| In vivo high-resolution magic angle spinning magnetic resonance spectroscopy of Drosophila melanogaster at 14.1 T shows trauma in aging and in innate immune-deficiency is linked to reduced insulin signaling. | |
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MedLine Citation:
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PMID: 20596596 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vivo magnetic resonance spectroscopy (MRS), a non-destructive biochemical tool for investigating live organisms, has yet to be used in the fruit fly Drosophila melanogaster, a useful model organism for investigating genetics and physiology. We developed and implemented a high-resolution magic-angle-spinning (HRMAS) MRS method to investigate live Drosophila at 14.1 T. We demonstrated, for the first time, the feasibility of using HRMAS MRS for molecular characterization of Drosophila with a conventional MR spectrometer equipped with an HRMAS probe. We showed that the metabolic HRMAS MRS profiles of injured, aged wild-type (wt) flies and of immune deficient (imd) flies were more similar to chico flies mutated at the chico gene in the insulin signaling pathway, which is analogous to insulin receptor substrate1-4 (IRS1-4) in mammals and less to those of adipokinetic hormone receptor (akhr) mutant flies, which have an obese phenotype. We thus provide evidence for the hypothesis that trauma in aging and in innate immune-deficiency is linked to insulin signaling. This link may explain the mitochondrial dysfunction that accompanies insulin resistance and muscle wasting that occurs in trauma, aging and immune system deficiencies, leading to higher susceptibility to infection. Our approach advances the development of novel in vivo non-destructive research approaches in Drosophila, suggests biomarkers for investigation of biomedical paradigms, and thus may contribute to novel therapeutic development. |
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Authors:
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Valeria Righi; Yiorgos Apidianakis; Dionyssios Mintzopoulos; Loukas Astrakas; Laurence G Rahme; A Aria Tzika |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of molecular medicine Volume: 26 ISSN: 1791-244X ISO Abbreviation: Int. J. Mol. Med. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-02 Completed Date: 2010-10-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9810955 Medline TA: Int J Mol Med Country: Greece |
Other Details:
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Languages: eng Pagination: 175-84 Citation Subset: IM |
Affiliation:
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NMR Surgical Laboratory, Department of Surgery, Massachusetts General Hospital and Shriners Burn Institute, Harvard Medical School, Boston, MA 02114, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Amino Acids / chemistry, metabolism Analysis of Variance Animals Drosophila Proteins / genetics* Drosophila melanogaster / metabolism* Immunologic Deficiency Syndromes Insulin / metabolism* Intracellular Signaling Peptides and Proteins / genetics Lipid Metabolism / physiology Lipids / chemistry Magnetic Resonance Spectroscopy / methods* Male Receptors, Glucagon / genetics Signal Transduction / physiology* Wounds and Injuries / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI063433/AI/NIAID NIH HHS; P50GM021700/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/AKHR protein, Drosophila; 0/Amino Acids; 0/Drosophila Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Lipids; 0/Receptors, Glucagon; 0/chico protein, Drosophila; 0/immune deficiency protein, Drosophila; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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