Document Detail

In vivo hemoglobin dosimetry of malonaldehyde and ethene in mice after induction of lipid peroxidation. Effects of membrane lipid fatty acid composition.
MedLine Citation:
PMID:  1670288     Owner:  NLM     Status:  MEDLINE    
Hemoglobin (Hb) adduct determination by the N-alkyl Edman method was used for in vivo dosimetry of endogenously formed malonaldehyde (MA) and ethene in mice fed diets with different fatty acid composition and induced for lipid peroxidation with carbon tetrachloride (CCl4). In order to amplify lipid peroxidation animals were pretreated with phenobarbital (PB) and the glutathione-depleting agent DL-buthionine-(S,R)-sulfoximine (BSO). Non-treated animals raised on different diets were used as controls. Lipid peroxidation products in liver were measured as 2-thiobarbituric acid reactive compounds (TBA-C). Livers from control mice fed a soya oil based diet (rich in polyunsaturated fatty acids, diet S) showed approximately 6.5-fold higher levels of TBA-C than those from animals raised on a coconut oil based diet (mostly saturated fatty acids, diet C). The level of adducts of MA to Hb, determined as N-(3-hydroxypropyl)valine, was approximately 1.5-fold higher in animals from diet S than in animals raised on diet C. The highest increases in the levels of TBA-C and MA adducts were obtained after a simultaneous treatment of the animals with PB, BSO and CCl4. The increases of TBA-C were 1.3-fold (diet C) and 1.7-fold (diet S). The corresponding increases of MA-Hb adduct levels were 1.3- and 1.6-fold respectively, indicating an increased susceptibility of mice fed diet S to lipid peroxidation. The level of adducts from ethene, determined as N-(2-hydroxyethyl)valine, was also higher in mice from diet S than in animals fed diet C, when all treatment groups were considered. The difference was, however, only slightly significant (P less than 0.02). No difference between control and CCl4-treated animals, with regard to the ethene-Hb adduct, was found. Our results validate the use of Hb dosimetry for monitoring the effects of factors known to influence lipid peroxidation induced in vivo.
A Kautiainen; M Törnqvist; B Anderstam; C E Vaca
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carcinogenesis     Volume:  12     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-07-12     Completed Date:  1991-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1097-102     Citation Subset:  IM    
Department of Radiobiology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
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MeSH Terms
Carbon Tetrachloride / pharmacology
Dietary Fats / pharmacology
Erythrocytes / metabolism
Ethylenes / metabolism*
Fatty Acids / analysis*
Glutathione / analysis
Hemoglobins / metabolism*
Lipid Peroxidation*
Liver / chemistry
Malondialdehyde / metabolism*
Membrane Lipids / analysis*
Mice, Inbred CBA
Thiobarbiturates / metabolism
Reg. No./Substance:
0/Dietary Fats; 0/Ethylenes; 0/Fatty Acids; 0/Hemoglobins; 0/Membrane Lipids; 0/Thiobarbiturates; 504-17-6/thiobarbituric acid; 542-78-9/Malondialdehyde; 56-23-5/Carbon Tetrachloride; 70-18-8/Glutathione; 74-85-1/ethylene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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