Document Detail


In vivo fluorescence microscopy of kidney subcapsular blood flow in mice. Effects of cyclosporine, (NVA2)-cyclosporine, and isradipine, a new calcium antagonist.
MedLine Citation:
PMID:  2972094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The subcapsular kidney microcirculation in mice was observed through a fluorescence microscope, recorded on videotape, and examined for response to infusions of cyclosporine A (CsA) and cyclosporine G (CsG). Coded video recordings were evaluated by a semiquantitative method. CsA infusion (1.6 +/- 0.4 mg/kg/min) induced a nearly complete inhibition of the subcapsular blood flow. At lower infusion rates (0.46 +/- 0.2 mg/kg/min), the blood flow inhibition was less pronounced. CsG infusions at corresponding rates induced significantly less inhibition. Pretreatment with a new calcium antagonist, isradipine (18-20 micrograms/kg bwt), completely prevented the CsA-induced impairment of subcapsular microcirculation. The calcium antagonist, however, did not improve blood flow when administered after induction of inhibition by CsA (16.8 +/- 2.5 mg/kg), emphasizing the importance of pretreatment. This study suggests hypoperfusion due to vasoconstriction as an important pathophysiologic mechanism for CsA-induced nephrotoxicity. CsG, when given at corresponding rates, induced less inhibition of the blood flow. Pretreatment with a calcium antagonist, isradipine, completely prevented a CsA-induced inhibition of blood flow, suggesting a potential value in the prevention of CsA-induced nephrotoxicity.
Authors:
P Rooth; I Dawidson; N Clothier; K Diller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Transplantation     Volume:  46     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-11-14     Completed Date:  1988-11-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  566-9     Citation Subset:  IM    
Affiliation:
Department of Surgery, University of Texas Southwestern Medical Center, Dallas 75235.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Channel Blockers / pharmacology
Cyclosporins / pharmacology
Female
Isradipine
Kidney Cortex / blood supply
Male
Mice
Microcirculation
Microscopy, Fluorescence
Premedication
Pyridines / administration & dosage,  pharmacology
Renal Circulation* / drug effects
Grant Support
ID/Acronym/Agency:
DK33470/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 0/Cyclosporins; 0/Pyridines; 75695-93-1/Isradipine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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