| In vivo fluorescence microscopy of blood flow in mouse pancreatic islets: adrenergic effects in lean and obese-hyperglycemic mice. | |
| | |
MedLine Citation:
|
PMID: 3900643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The microcirculation in the islets of Langerhans was examined by fluorescence microscopy in living mice injected with fluorescent dextran. The islet capillary network was denser and more tortuously arranged in obese-hyperglycemic (ob/ob) mice than in lean controls. Injection of norepinephrine (0.5-4.0 micrograms/kg body wt) immediately led to a pronounced inhibition of islet blood flow in ob/ob mice. In experiments with lean mice less striking effects were seen. With as high a dose as 20 micrograms norepinephrine/kg body wt only a slight retardation and very brief stop of the flow occurred. The inhibition in ob/ob mice was blocked by phentolamine, indicating that the norepinephrine-induced inhibition was mediated by alpha-adrenoceptors. The alpha-2-adrenoceptor agonist, clonidine, had no effect on islet blood flow, suggesting that the effect of norepinephrine was due to alpha-1-adrenoceptor stimulation. It is concluded that in the living animal norepinephrine inhibits insulin secretion from the pancreas by a twofold mechanism involving inhibition of exocytosis (alpha-2-receptors on the beta-cells) as well as retardation of blood flow (alpha-1-receptors on blood vessels). |
| | |
Authors:
|
P Rooth; K Grankvist; I B Täljedal |
Related Documents
:
|
1553373 - Dissociation between the blood flow response of transplanted pancreatic islets and that... 14527913 - Intra-arterial infusion chemotherapy with angiotensin-ii for locally advanced and nonre... 8917183 - Cerebral hypoperfusion in orthostatic hypotension with globally denervated myocardium. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Microvascular research Volume: 30 ISSN: 0026-2862 ISO Abbreviation: Microvasc. Res. Publication Date: 1985 Sep |
Date Detail:
|
Created Date: 1985-11-14 Completed Date: 1985-11-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0165035 Medline TA: Microvasc Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 176-84 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Clonidine / pharmacology* Female Islets of Langerhans / anatomy & histology, blood supply*, drug effects Mice Mice, Obese Microscopy, Fluorescence Norepinephrine / pharmacology* Phentolamine / pharmacology* Regional Blood Flow / drug effects |
| Chemical | |
Reg. No./Substance:
|
4205-90-7/Clonidine; 50-60-2/Phentolamine; 51-41-2/Norepinephrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation of genes from Candida albicans by complementation in Saccharomyces cerevisiae.
Next Document: Characterization of pathogenic constituents of Cryptococcus neoformans strains.