| In vivo fate of HIV-1-infected T cells: quantitative analysis of the transition to stable latency. | |
| | |
MedLine Citation:
|
PMID: 7489410 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Although it is presumed that the integration of HIV-1 into the genome of infected CD4+ T lymphocytes allows viral persistence, there has been little direct evidence that CD4+ T cells with integrated provirus function as a latent reservoir for HIV-1 in infected individuals. Using resting CD4+ T-cell populations of extremely high purity and a novel assay that selectively and unambiguously detects integrated HIV-1, we show that resting CD4+ T cells harbouring integrated provirus are present in some infected individuals. However, these cells do not accumulate within the circulating pool of resting CD4+ T cells in the early stages of HIV-1 infection and do not accumulate even after prolonged periods in long-term survivors of HIV-1 infection. These results suggest that because of viral cytopathic effects and/or host effector mechanisms, productively infected CD4+ T cells do not generally survive for long enough to revert to a resting memory state in vivo. |
| | |
Authors:
|
T W Chun; D Finzi; J Margolick; K Chadwick; D Schwartz; R F Siliciano |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Nature medicine Volume: 1 ISSN: 1078-8956 ISO Abbreviation: Nat. Med. Publication Date: 1995 Dec |
Date Detail:
|
Created Date: 1996-01-02 Completed Date: 1996-01-02 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9502015 Medline TA: Nat Med Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1284-90 Citation Subset: IM; X |
Affiliation:
|
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence CD4-Positive T-Lymphocytes / virology* Cell Separation DNA Primers DNA, Viral / analysis* HIV Infections / blood, virology* HIV-1 / genetics*, isolation & purification Humans Molecular Sequence Data Polymerase Chain Reaction Proviruses / genetics* Virus Integration Virus Latency |
| Grant Support | |
ID/Acronym/Agency:
|
AI23871/AI/NIAID NIH HHS; AI28108/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/DNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetic control of the CD4/CD8 T-cell ratio in humans.
Next Document: The Swedish mutation causes early-onset Alzheimer's disease by beta-secretase cleavage within the se...