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In vivo extraction of Arabidopsis cell turgor pressure using nanoindentation in conjunction with finite element modeling.
MedLine Citation:
PMID:  23036157     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Turgor pressure in plant cells is involved in many important processes. While stable and normal turgor pressure is required for healthy growth of a plant, changes in turgor pressure are indicative of changes taking place within the plant tissue. The ability to quantify turgor pressure of plant cells in vivo would provide opportunities to understand better the process of pressure regulation within plants, especially when plant stress is considered, and to understand the role of turgor pressure in cellular signaling. Current experimental methods do not separate the influence of the turgor pressure from the effects associated with the deformation of the cell wall when estimates of turgor pressure are made. In this article, nanoindentation measurements are combined with finite element simulations to determine the turgor pressure of cells in vivo while explicitly separating the cell wall properties from the turgor pressure effects. Quasi-static cyclic tests with variable depth form the basis of the measurements while relaxation tests at low depth are used to determine the viscoelastic material properties of the cell wall. Turgor pressure is quantified using measurements on Arabidopsis thaliana in three different pressure states (control, turgid and plasmolyzed) and at various stages of plant development. These measurements can be performed on cells in vivo without invoking damage to the cells such that pressure changes can be studied for a variety of conditions to provide new insights into the biological response to different plant stress conditions. © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Authors:
Elham Forouzesh; Ashwani Goel; Sally A Mackenzie; Joseph A Turner
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Affiliation:
Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, W342 Nebraska Hall, Lincoln, Nebraska, 68588-0526, USA.
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