Document Detail

In vivo expression profile of an endothelial nitric oxide synthase promoter-reporter transgene.
MedLine Citation:
PMID:  10749733     Owner:  NLM     Status:  MEDLINE    
Endothelium-derived nitric oxide (NO) is primarily attributable to constitutive expression of the endothelial nitric oxide synthase (eNOS) gene. Although a more comprehensive understanding of transcriptional regulation of eNOS is emerging with respect to in vitro regulatory pathways, their relevance in vivo warrants assessment. In this regard, promoter-reporter insertional transgenic murine lines were created containing 5,200 bp of the native murine eNOS promoter directing transcription of nuclear-localized beta-galactosidase. Examination of beta-galactosidase expression in heart, lung, kidney, liver, spleen, and brain of adult mice demonstrated robust signal in large and medium-sized blood vessels. Small arterioles, capillaries, and venules of the microvasculature were notably negative, with the exception of the vasa recta of the medullary circulation of the kidney, which was strongly positive. Only in the brain was the reporter expressed in non-endothelial cell types, such as the CA1 region of the hippocampus. Epithelial cells of the bronchi, bronchioles, and alveoli were scored as negative, as was renal tubular epithelium. Cardiac myocytes, skeletal muscle, and smooth muscle of both vascular and nonvascular sources failed to demonstrate beta-galactosidase staining. Expression was uniform across multiple founders and was not significantly affected by genomic integration site. These transgenic eNOS promoter-reporter lines will be a valuable resource for ongoing studies addressing the regulated expression of eNOS in vivo in both health and disease.
A M Teichert; T L Miller; S C Tai; Y Wang; X Bei; G B Robb; M J Phillips; P A Marsden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  278     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-04-27     Completed Date:  2000-04-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1352-61     Citation Subset:  IM    
Department of Medicine, St. Michael's Hospital and University of Toronto, Toronto M5S 1A8, Ontario, Canada M5S 1X8.
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MeSH Terms
Arteriosclerosis / enzymology
Endothelium, Vascular / enzymology
Gene Expression Regulation, Enzymologic*
Genes, Reporter
Hypertension / enzymology
Juxtaglomerular Apparatus / enzymology
Kidney / blood supply,  physiology
Lac Operon
Mice, Inbred C57BL
Mice, Transgenic
Mutagenesis, Insertional
Nephrons / enzymology
Nitric Oxide Synthase / genetics*
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Promoter Regions, Genetic / genetics*
RNA, Messenger / analysis
Renal Circulation / physiology
Transgenes / genetics*
Reg. No./Substance:
0/RNA, Messenger; EC Oxide Synthase; EC Oxide Synthase Type II; EC Oxide Synthase Type III; EC protein, mouse

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