Document Detail

In vivo evidence for the crucial role of SF1 in steroid-producing cells of the testis, ovary and adrenal gland.
MedLine Citation:
PMID:  23136395     Owner:  NLM     Status:  MEDLINE    
Adrenal and gonadal steroids are essential for life and reproduction. The orphan nuclear receptor SF1 (NR5A1) has been shown to regulate the expression of enzymes involved in steroid production in vitro. However, the in vivo role of this transcription factor in steroidogenesis has not been elucidated. In this study, we have generated steroidogenic-specific Cre-expressing mice to lineage mark and delete Sf1 in differentiated steroid-producing cells of the testis, the ovary and the adrenal gland. Our data show that SF1 is a regulator of the expression of steroidogenic genes in all three organs. In addition, Sf1 deletion leads to a radical change in cell morphology and loss of identity. Surprisingly, sexual development and reproduction in mutant animals were not compromised owing, in part, to the presence of a small proportion of SF1-positive cells. In contrast to the testis and ovary, the mutant adult adrenal gland showed a lack of Sf1-deleted cells and our studies suggest that steroidogenic adrenal cells during foetal stages require Sf1 to give rise to the adult adrenal population. This study is the first to show the in vivo requirements of SF1 in steroidogenesis and provides novel data on the cellular consequences of the loss of this protein specifically within steroid-producing cells.
F William Buaas; Jennifer R Gardiner; Sally Clayton; Pierre Val; Amanda Swain
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-07
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-22     Completed Date:  2013-01-31     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  4561-70     Citation Subset:  IM    
Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
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MeSH Terms
Adrenal Glands / cytology,  embryology,  growth & development,  metabolism*
Bacterial Proteins / genetics,  metabolism
Cholesterol Side-Chain Cleavage Enzyme / genetics
DNA-Binding Proteins / genetics,  metabolism,  physiology*
Embryo, Mammalian
Gene Deletion
Gonadal Steroid Hormones / metabolism*
Integrases / genetics
Luminescent Proteins / genetics,  metabolism
Mice, Transgenic
Ovary / cytology,  embryology,  growth & development,  metabolism*
Testis / cytology,  embryology,  growth & development,  metabolism*
Transcription Factors / genetics,  metabolism,  physiology*
Transgenes / genetics
Grant Support
BB/C517825/1//Biotechnology and Biological Sciences Research Council; G0901645//Medical Research Council
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Gonadal Steroid Hormones; 0/Luminescent Proteins; 0/Transcription Factors; 0/Zfp162 protein, mouse; 0/yellow fluorescent protein, Bacteria; EC Side-Chain Cleavage Enzyme; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases

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