Document Detail

In vivo evaluation of the genotoxic effects of clomiphene citrate on rat reticulocytes: a micronucleus genotoxicity.
MedLine Citation:
PMID:  16508321     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the genotoxic effects of clomiphene citrate (CC) on rat reticulocytesin vivo. METHODS: In this prospective, randomized, controlled study, rats were each assigned randomly to the CC 50, CC 100, CC 200, or control group and were given repeat doses of 0.16, 0.32 or 0.64 mg CC, or normal saline, respectively. Each study group received its CC dose in 2 ml of saline intraperitoneally for 5 days, while the control group received only 2 ml of saline. Each treatment cycle was repeated six times. Six months later, the rats were euthanized. Bone marrow tissues were removed, and pluripotent reticulocyte cells with micronuclei, nuclear buds, and binuclear abnormalities were analyzed using an in situmicronuclei assay under light microscopy. The proportion of micronucleated erythrocytes was measured. RESULTS: Fewer cells with nuclear buds and binuclear abnormalities were detected in the CC 50 group and controls. The CC 100 and 200 groups had significantly (p < 0.05) more nuclear buds and binuclear abnormalities compared with the CC 50 group and controls in the cytogenetic analysis of bone marrow stem cells. CONCLUSION: In rats, the micronucleus genotoxicity assay suggests a dose-dependent CC effect on genomic instability in bone marrow stem cells in vivo.
Bulent Duran; Ismail Ozdemir; Yeltekin Demirel; Ozturk Ozdemir; Ali Cetin; Aysel Guven
Publication Detail:
Type:  Journal Article     Date:  2006-02-23
Journal Detail:
Title:  Gynecologic and obstetric investigation     Volume:  61     ISSN:  0378-7346     ISO Abbreviation:  Gynecol. Obstet. Invest.     Publication Date:  2006  
Date Detail:
Created Date:  2006-05-15     Completed Date:  2006-10-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7900587     Medline TA:  Gynecol Obstet Invest     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  228-31     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2006 S. Karger AG, Basel.
Department of Obstetrics and Gynecology, Cumhuriyet University, Faculty of Medicine, Sivas, Turkey.
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MeSH Terms
Clomiphene / toxicity*
Dose-Response Relationship, Drug
Fertility Agents, Female / toxicity*
Micronuclei, Chromosome-Defective / chemically induced*
Micronucleus Tests
Rats, Wistar
Reticulocytes / drug effects*,  pathology*
Stem Cells / drug effects
Reg. No./Substance:
0/Fertility Agents, Female; 911-45-5/Clomiphene

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