Document Detail


In vivo digestion of infant formula in piglets: protein digestion kinetics and release of bioactive peptides.
MedLine Citation:
PMID:  22377314     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The first months of life correspond to a key period in human life where dramatic physiological changes (establishment of microbiota, development of the immune system, etc.) occur. In order to better control these changes it is necessary to understand the behaviour of food in the gastrointestinal tract of the newborn. Infant formula is the only food for the newborn when breast-feeding is impossible. The kinetics of digestion of milk proteins and the nature of the peptides liberated in the small intestine throughout infant formula digestion have never been extensively investigated so far and were therefore studied using the piglet as a model of the newborn child. Piglets were fed infant formula by an automatic delivery system during 28 d, and slaughtered 30, 90 and 210 min after the last meal. Contents of stomach, proximal and median jejunum and ileum were collected and characterised. The extent of β-lactoglobulin (β-lg), α-lactalbumin (α-la) and casein proteolysis was monitored by inhibition ELISA, SDS-PAGE, immunoblotting and MS. At 30 min after the last meal, caseins were shown to be extensively hydrolysed in the stomach. Nevertheless, peptides originating mainly from β-caseins (from 509 to 2510 Da) were identified in the jejunum and ileum of the piglets. β-Lg partially resisted gastric digestion but completely disappeared in the stomach after 210 min. α-La had a similar behaviour to that of β-lg. Two large peptides (4276 and 2674 Da) generated from β-lg were present in the ileum after 30 and 210 min and only one (2674 Da) after 90 min.
Authors:
Karima Bouzerzour; François Morgan; Isabelle Cuinet; Cécile Bonhomme; Julien Jardin; Isabelle Le Huërou-Luron; Didier Dupont
Related Documents :
7595774 - Use of a truss to maintain inguinal hernia reduction in a very low birth weight infant.
16509904 - Fetal surgery for congenital diaphragmatic hernia.
7012924 - Delayed appearance of right diaphragmatic hernia associated with group b streptococcal ...
3529934 - Infant botulism. three cases in a small town.
1846934 - Antagonism of marihuana effects by indomethacin in humans.
19709094 - Psychopathology among preterm infants using the diagnostic classification zero to three.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-1
Journal Detail:
Title:  The British journal of nutrition     Volume:  -     ISSN:  1475-2662     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-10     Citation Subset:  -    
Affiliation:
INRA, UMR 1253, STLO, Rennes, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  [System biology and synthetic biology modify drug discovery and development].
Next Document:  Chromatin structural changes around satellite repeats on the female sex chromosome in Schistosoma ma...