Document Detail


In vivo determination of rumen undegradable protein of dried distillers grains with solubles and evaluation of duodenal microbial crude protein flow.
MedLine Citation:
PMID:  23148253     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The objectives of this trial were to determine the rumen undegradable protein (RUP) of dried distillers grains with solubles (DDGS), to compare the estimates of duodenal bacterial crude protein (BCP) flow using diaminopumelic acid (DAPA) or DNA as bacterial markers, and to estimate duodenal protozoal crude protein (PCP) and yeast crude protein (YCP) flow when DDGS are fed. Three crossbred steers fitted with ruminal and double L-shaped duodenal cannulae (average BW 780 ± 137 kg) were used in a 3 treatment, 6 period cross-over design. Animals were housed in individual free stalls and fed twice daily at 0700 and 1900. Diets (DM basis) were 1) CONTROL, 0% DDGS, but with 19.5% corn bran, 20% sorghum silage, 60% brome hay, 0.5% trace minerals and 0.25% urea; 2) LOW DDGS, inclusion of 9.75% DDGS replacing equal percentage of corn bran; 3) HIGH DDGS, inclusion of 19.5% DDGS completely replacing corn bran. Duodenal BCP flow was estimated using DAPA and DNA as bacterial markers. In addition, duodenal PCP and YCP flow were estimated using DNA markers. The value of DDGS RUP as a percent of CP was determined to be 63.0 ± 0.64%. Estimates of duodenal BCP flow using DAPA were 473, 393, 357 ± 78 g/d (P = 0.09) for CONTROL, LOW DDGS and HIGH DDGS, respectively. Estimates of duodenal BCP flow using DNA were 479, 397 and 368 ± 74 g/d (P = 0.14), respectively. Average BCP flow across treatments was unaffected (P = 0.71) by marker type and were 404 and 417 ± 83 g/d for DAPA and DNA markers, respectively. Estimates of duodenal PCP flow were 82, 80 and 78 ± 12 g/d (P = 0.64) for CONTROL, LOW DDGS and HIGH DDGS, respectively. Estimates of duodenal YCP flow were 0.15, 1.94 and 4.80 ± 0.66 g/d (P < 0.01) for CONTROL, LOW DDGS and HIGH DDGS, respectively. Duodenal BCP flow tended to decrease with DDGS inclusion, but estimates were not affected by marker type. In addition, DDGS did not affect duodenal PCP supply and provided small amounts of duodenal YCP. Overall, the value of DDGS RUP determined in this study will contribute to better understand the effect of this byproduct in ruminant nutrition.
Authors:
E Castillo-Lopez; T J Klopfenstein; S C Fernando; P J Kononoff
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-12
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Animal Science, University of Nebraska-Lincoln, Lincoln, Nebraska, 68583-0908.
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