Document Detail

In vivo detection of basal cell carcinoma: comparison of a reflectance confocal microscope and a multiphoton tomograph.
MedLine Citation:
PMID:  23456144     Owner:  NLM     Status:  MEDLINE    
The standard diagnostic procedure for basal cell carcinoma (BCC) is invasive tissue biopsy with time-consuming histological examination. To reduce the number of biopsies, noninvasive optical methods have been developed providing high-resolution skin examination. We present direct comparison of a reflectance confocal microscope (RLSM) and a multiphoton tomograph (MPT) for BCC diagnosis. Both systems are applied to nine patients prior to surgery, and the results are analyzed, including histological results. Both systems prove suitable for detecting typical characteristics of BCC in various stages. The RLSM allows large horizontal overview images to be obtained, enabling the investigator to find the regions of interest quickly, e.g., BCC nests. Elongated cells and palisading structures are easily recognized using both methods. Due to the higher resolution, changes in nucleus diameter or cytoplasm could be visualized with the MPT. Therefore, the nucleus diameter, nucleus/cytoplasm ratio, and cell density are estimated for normal and BCC cells using the MPT. The nucleus of elongated BCC cells is significantly longer than other measured normal skin cells, whereas the cell density and nucleus/cytoplasm ratio of BCC cannot be significantly distinguished from granular cells.
Martina Ulrich; Marisa Klemp; Maxim E Darvin; Karsten König; Jürgen Lademann; Martina C Meinke
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  18     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-03-04     Completed Date:  2013-06-06     Revised Date:  2013-08-06    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  61229     Citation Subset:  IM    
Department of Dermatology, Allergology and Venerology, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
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MeSH Terms
Aged, 80 and over
Carcinoma, Basal Cell / diagnosis*,  pathology
Cell Count
Cell Nucleus / pathology
Cell Nucleus Shape
Cell Nucleus Size
Cytoplasm / pathology
Microscopy, Confocal / methods*
Microscopy, Fluorescence, Multiphoton / methods*
Middle Aged
Optical Phenomena
Skin Neoplasms / diagnosis*,  pathology
Tomography, Optical / methods*

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