| In vivo cultivation of tumor cells in hollow fibers. | |
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MedLine Citation:
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PMID: 7603295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Advancement of potential anti-cancer agents from "discovery" in an in vitro screen to pre-clinical development requires a demonstration of in vivo efficacy in one or more animal models of neoplastic disease. Most such models require considerable materials in terms of laboratory animals and test compound as well as substantial amounts of time (and cost) to determine whether a given experimental agent or series of agents have even minimal anti-tumor activity. The present study was initiated to assess the feasibility of employing an alternate methodology for preliminary in vivo evaluations of therapeutic efficacy. Results of experimentation to date demonstrate that a hollow fiber encapsulation/implantation methodology provides quantitative indices of drug efficacy with minimum expenditures of time and materials. Following further pharmacologic calibrations, the hollow fiber technique is anticipated (a) to identify compounds having moderate to prominent anti-cancer activity and (b) to facilitate the identification of sensitive tumor cell line "targets" and optimal or near-optimal treatment regimens for subsequent testing using standard in vivo solid tumor models. The potential suitability of this methodology is demonstrated with several standard anti-neoplastic agents. |
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Authors:
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M G Hollingshead; M C Alley; R F Camalier; B J Abbott; J G Mayo; L Malspeis; M R Grever |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Life sciences Volume: 57 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 1995 |
Date Detail:
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Created Date: 1995-08-07 Completed Date: 1995-08-07 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 131-41 Citation Subset: IM |
Affiliation:
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Biological Testing Branch, National Cancer Institute, NCI-FCRDC, Frederick, MD 21702, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / pharmacology Cell Communication / physiology Cell Division / physiology Cytological Techniques Drug Screening Assays, Antitumor Female Humans Male Mice Mice, Nude Neoplasm Transplantation Neoplasms / drug therapy, pathology* Polymers* Polyvinyls* Sulfones* Transplantation, Heterologous Tumor Cells, Cultured* / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Polymers; 0/Polyvinyls; 0/Sulfones; 24937-79-9/polyvinylidene fluoride; 25135-51-7/polysulfone P 1700 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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