Document Detail


In vivo control of gluconeogenesis in wild-type Neurospora crassa and in the adenylate cyclase-deficient cr-1 (crisp) mutant.
MedLine Citation:
PMID:  2522093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rate of cycloheximide-resistant incorporation of carbon from [14C]alanine and [14C]acetate into polysaccharidic material was used to study gluconeogenic activity in wild-type Neurospora crassa and in the adenylate cyclase-deficient cr-1 (crisp-1) mutant. The wild-type efficiently utilized alanine and acetate as gluconeogenic substrates, whereas the mutant used acetate efficiently but was unable to use alanine. Cycloheximide-resistant 14C-incorporating activity was sensitive to carbon catabolite effects (repression and inactivation) in the two strains, which suggested that cyclic AMP metabolism was not involved in these regulatory responses. In the wild type, gluconeogenesis was induced by incubation of the cells in the absence of a carbon source. In contrast, cr-1 required supplementation with acetate. This finding suggested that induction of gluconeogenesis in N. crassa could be mediated by metabolites formed in carbon-starved cells. The cr-1 mutant seemed to be deficient in this process and to depend on an exogenous effector to induce gluconeogenesis. Incubation of cr-1 with cyclic AMP partially overcame the acetate requirement for induction of gluconeogenesis.
Authors:
M J Neves; H F Terenzi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  171     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-04-14     Completed Date:  1989-04-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1767-71     Citation Subset:  IM    
Affiliation:
Departamentos de Fisiologia e Bioquímica, Faculdade de Medicina, Universidade de São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Acetates / metabolism
Adenylate Cyclase / genetics*
Alanine / metabolism
Cycloheximide / pharmacology
Drug Resistance
Gluconeogenesis*
Kinetics
Mutation*
Neurospora / metabolism*
Neurospora crassa / drug effects,  genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Acetates; 56-41-7/Alanine; 66-81-9/Cycloheximide; EC 4.6.1.1/Adenylate Cyclase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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