Document Detail


In vivo chlorine-35, sodium-23 and proton magnetic resonance imaging of the rat brain.
MedLine Citation:
PMID:  20232452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we demonstrate the feasibility of combined chlorine-35, sodium-23 and proton magnetic resonance imaging (MRI) at 9.4 Tesla, and present the first in vivo chlorine-35 images obtained by means of MRI. With the experimental setup used in this study all measurements could be done in one session without changing the setup or moving the subject. The multinuclear measurement requires a total measurement time of 2 h and provides morphological (protons) and physiological (sodium-23, chlorine-35) information in one scanning session. Chlorine-35, sodium-23 and high resolution proton images were acquired from a phantom, a healthy rat and from a rat displaying a focal cerebral infarction. Compared to the healthy tissue a signal enhancement of a factor of 2.2 +/- 0.2 in the chlorine-35 and a factor of 2.9 +/- 0.6 in the sodium-23 images is observed in the areas of infarction. Exemplary unlocalized measurement of the in vivo longitudinal and transversal relaxation time of chlorine-35 in a healthy rat showed multi-exponential behaviour. A biexponential fit revealed a fast and a slow relaxing component with T(1,a) = (1.7 +/- 0.4) ms, T(1,b) = (25.1 +/- 1.4) ms, amplitudes of A = 0.26 +/- 0.02, (1-A) = 0.74 +/- 0.02 and T(2,a) = (1.3 +/- 0.1) ms, T(2,b) = (11.8 +/- 1.1) ms, A = 0.64 +/- 0.02, (1-A) = 0.36 +/- 0.02. Combined proton, sodium-23 and chlorine-35 MRI may provide a new approach for non-invasive studies of ionic regulatory processes under physiological and pathological conditions in vivo.
Authors:
Stefan Kirsch; Mark Augath; David Seiffge; Lothar Schilling; Lothar R Schad
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  NMR in biomedicine     Volume:  23     ISSN:  1099-1492     ISO Abbreviation:  NMR Biomed     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-27     Completed Date:  2010-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8915233     Medline TA:  NMR Biomed     Country:  England    
Other Details:
Languages:  eng     Pagination:  592-600     Citation Subset:  IM    
Affiliation:
Department of Computer Assisted Clinical Medicine, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany. stefan.kirsch@medma.uni-heidelberg.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / anatomy & histology*,  metabolism*,  pathology
Brain Infarction / metabolism,  pathology
Chlorine / metabolism*
Magnetic Resonance Imaging / instrumentation,  methods*
Protons*
Rats
Rats, Sprague-Dawley
Sodium / metabolism*
Chemical
Reg. No./Substance:
0/Protons; 7440-23-5/Sodium; 7782-50-5/Chlorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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