| In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore. | |
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MedLine Citation:
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PMID: 20735485 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neocarzinostatin (NCS) is an enediyne antibiotic produced by Streptomyces carzinostaticus. The NCS chromophore consists of an enediyne core, a sugar moiety, and a naphthoic acid (NA) moiety. The latter plays a key role in binding the NCS chromophore to its apoprotein to protect and stabilize the bioactive NCS chromophore. In this study, we expressed three genes: ncsB (naphthoic acid synthase), ncsB3 (P450 hydroxylase), and ncsB1 (O-methyltransferase), in Streptomyces lividans TK24. The three genes were sufficient to produce 2-hydroxy-7-methoxy-5-methyl-1-naphthoic acid. Production was analyzed and confirmed by LC-MS and nuclear magnetic resonance. Here, we report the functional characterization of ncsB3 and thereby elucidate the complete biosynthetic pathway of NA moiety of the NCS chromophore. |
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Authors:
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Vu Thi Thu Hang; Tae Jin Oh; Tokutaro Yamaguchi; Jae Kyung Sohng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-23 |
Journal Detail:
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Title: FEMS microbiology letters Volume: 311 ISSN: 1574-6968 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-23 Completed Date: 2011-01-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
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Languages: eng Pagination: 119-25 Citation Subset: IM |
Copyright Information:
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© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Asansi, Chungnam, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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biosynthesis*,
chemistry Bacterial Proteins / genetics, metabolism* Biosynthetic Pathways Carboxylic Acids / chemistry, metabolism* Cytochrome P-450 Enzyme System / genetics, metabolism* Gene Expression Regulation, Bacterial Molecular Structure Naphthalenes / chemistry, metabolism* Streptomyces / chemistry, enzymology, metabolism* Zinostatin / analogs & derivatives, biosynthesis, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Carboxylic Acids; 0/Naphthalenes; 81604-85-5/neocarzinostatin chromophore; 86-55-5/1-naphthoic acid; 9014-02-2/Zinostatin; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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