Document Detail


In vivo cardiovascular responses to isoproterenol, dopamine and tyramine after prolonged infusion of isoproterenol.
MedLine Citation:
PMID:  6094797     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effects of prolonged in vivo infusion of isoproterenol on acute cardiovascular responses to isoproterenol, dopamine and tyramine were studied in pithed rats. Isoproterenol infusion resulted in a significant decrease in control values for maximum left ventricular dP/dt; heart rate and left ventricular systolic blood pressure were not altered. This treatment also depleted both atrial and ventricular stores of norepinephrine and caused cardiac hypertrophy. Isoproterenol infusion resulted in a desensitization of drug-induced cardiovascular responses. The acute in vivo effects of isoproterenol on maximum left ventricular dP/dt, heart rate and left ventricular systolic blood pressure responses to isoproterenol were severely attenuated. The ED50 for maximum left ventricular dP/dt was increased 36-fold and maximal responses were reduced by half; changes in heart rate occurred in a parallel fashion. By contrast, ED50 values for inotropic responses to tyramine and dopamine were increased 14- and 4-fold, respectively, whereas increases in heart rate were blunted. Tyramine and dopamine-mediated increases in heart rate were completely attenuated by desensitization; chronotropic effects were again evident after pretreatment with the selective alpha-1 blocker prazosin. In addition, prazosin blocked the inotropic responses to tyramine and dopamine after desensitization and this antagonism was only slightly enhanced by addition of propranolol (prazosin + propranolol); propranolol alone was ineffective. These results are consistent with the down-regulation of beta adrenoceptors after prolonged exposure to catecholamines and indicate that under such conditions the alpha-mediated cardiovascular responses may be unmasked. Compared to pure beta agonists, agents with a degree of alpha-1 activity might be superior inotropes in heart failure patients who characteristically present with depleted stores of myocardial norepinephrine and minimal beta adrenoceptor reserve.
Authors:
J S Hayes; G D Pollock; R W Fuller
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  231     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-01-22     Completed Date:  1985-01-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  633-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight / drug effects
Cardiovascular System / drug effects*
Catecholamines / analysis
Dopamine / pharmacology*
Isoproterenol / administration & dosage,  blood,  pharmacology*
Male
Myocardium / analysis
Rats
Rats, Inbred Strains
Receptors, Adrenergic, alpha / physiology
Receptors, Adrenergic, beta / drug effects
Tyramine / pharmacology*
Chemical
Reg. No./Substance:
0/Catecholamines; 0/Receptors, Adrenergic, alpha; 0/Receptors, Adrenergic, beta; 51-67-2/Tyramine; 7683-59-2/Isoproterenol

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