Document Detail


In vivo bioluminescent imaging of irradiated orthotopic pancreatic cancer xenografts in nonobese diabetic-severe combined immunodeficient mice: a novel method for targeting and assaying efficacy of ionizing radiation.
MedLine Citation:
PMID:  20563256     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Adenocarcinoma of the pancreas is a lethal malignancy, and better models to study tumor behavior in vivo are needed for the development ofmore effective therapeutics. Ionizing radiation is a treatment modality that is commonly used in the clinical setting, in particular, for locally confined disease; however, good model systems to study the effect of ionizing radiation in orthotopic tumors have not been established. In an attempt to create clinically relevant models for studying treatments directed against pancreatic cancer, we have defined a methodology to measure the effect of varying doses of radiation in established human pancreatic cancer orthotopic xenografts using two different pancreatic cancer cell lines (Panc-1 and BXPC3) infected with a lentiviral vector expressing CMV promoter-driven luciferase to allow bioluminescence imaging of live animals in real time. Quantifiable photon emission from luciferase signaling in vivo correlated well with actual tumor growth. Bioluminescence imaging of the established pancreatic xenografts was used to direct delivery of radiation to the orthotopic tumors and minimize off-target adverse effects. Growth delay was observed with schedules in the range of 7.5 Gy in five fractions to 10 Gy in four fractions, whereas doses 3 Gy or higher produced toxic adverse effects. In conclusion, we describe a model in which the effects of ionizing radiation, alone or in combination with other therapeutics, in orthotopic xenografts, can be studied.
Authors:
Cheong J Lee; Aaron C Spalding; Edgar Ben-Josef; Lidong Wang; Diane M Simeone
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Publication Detail:
Type:  Journal Article     Date:  2010-06-01
Journal Detail:
Title:  Translational oncology     Volume:  3     ISSN:  1936-5233     ISO Abbreviation:  Transl Oncol     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-21     Completed Date:  2011-07-14     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  101472619     Medline TA:  Transl Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-9     Citation Subset:  -    
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Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
R01 CA174836/CA/NCI NIH HHS
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