Document Detail


In vivo bioluminescence imaging of cell differentiation in biomaterials: a platform for scaffold development.
MedLine Citation:
PMID:  23013334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vivo testing is a mandatory last step in scaffold development. Agile longitudinal noninvasive real-time monitoring of stem cell behavior in biomaterials implanted in live animals should facilitate the development of scaffolds for tissue engineering. We report on a noninvasive bioluminescence imaging (BLI) procedure for simultaneous monitoring of changes in the expression of multiple genes to evaluate scaffold performance in vivo. Adipose tissue-derived stromal mensenchymal cells were dually labeled with Renilla red fluorescent protein and firefly green fluorescent protein chimeric reporters regulated by cytomegalovirus and tissue-specific promoters, respectively. Labeled cells were induced to differentiate in vitro and in vivo, by seeding in demineralized bone matrices (DBMs) and monitored by BLI. Imaging results were validated by RT-polymerase chain reaction and histological procedures. The proposed approach improves molecular imaging and measurement of changes in gene expression of cells implanted in live animals. This procedure, applicable to the simultaneous analysis of multiple genes from cells seeded in DBMs, should facilitate engineering of scaffolds for tissue repair.
Authors:
Juli R Bagó; Elisabeth Aguilar; Maria Alieva; Carolina Soler-Botija; Olaia F Vila; Silvia Claros; José A Andrades; José Becerra; Nuria Rubio; Jerónimo Blanco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-21
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  19     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-04     Completed Date:  2013-09-25     Revised Date:  2014-03-09    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  593-603     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD31 / metabolism
Biocompatible Materials / pharmacology*
Bone Matrix / metabolism
Cell Differentiation / drug effects*,  genetics
Cell Hypoxia / drug effects,  genetics
Endothelial Cells / cytology,  drug effects,  metabolism
Fluorescent Antibody Technique
Genes, Reporter / genetics
Humans
Luciferases / metabolism
Luminescent Measurements / methods*
Mice
Mice, SCID
Neovascularization, Physiologic / drug effects,  genetics
Osteocalcin / genetics,  metabolism
Osteogenesis / drug effects,  genetics
Response Elements / genetics
Tissue Scaffolds / chemistry*
Chemical
Reg. No./Substance:
0/Antigens, CD31; 0/Biocompatible Materials; 104982-03-8/Osteocalcin; EC 1.13.12.-/Luciferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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