Document Detail


In vivo biocompatibility and mechanical properties of porous zein scaffolds.
MedLine Citation:
PMID:  17582490     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In our previous study, a three-dimensional zein porous scaffold with a compressive Young's modulus of up to 86.6+/-19.9 MPa and a compressive strength of up to 11.8+/-1.7 MPa was prepared, and was suitable for culture of mesenchymal stem cells (MSCs) in vitro. In this study, we examined its tissue compatibility in a rabbit subcutaneous implantation model; histological analysis revealed a good tissue response and degradability. To improve its mechanical property (especially the brittleness), the scaffolds were prepared using the club-shaped mannitol as the porogen, and stearic acid or oleic acid was added. The scaffolds obtained had an interconnected tubular pore structure, 100-380 microm in pore size, and about 80% porosity. The maximum values of the compressive strength and modulus, the tensile strength and modulus, and the flexural strength and modulus were obtained at the lowest porosity, reaching 51.81+/-8.70 and 563.8+/-23.4 MPa; 3.91+/-0.86 and 751.63+/-58.85 MPa; and 17.71+/-3.02 and 514.39+/-19.02 MPa, respectively. Addition of 15% stearic acid or 20% oleic acid did not affect the proliferation and osteogenic differentiation of MSCs, and a successful improvement of mechanical properties, especially the brittleness of the zein scaffold could be achieved.
Authors:
Hua-Jie Wang; Sheng-Ju Gong; Zhi-Xin Lin; Jian-Xi Fu; Song-Tao Xue; Jing-Chun Huang; Jin-Ye Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-19
Journal Detail:
Title:  Biomaterials     Volume:  28     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3952-64     Citation Subset:  IM    
Affiliation:
College of Life Science and Biotechnology, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry*,  pharmacology*
Elasticity
Materials Testing
Mechanics
Porosity
Rabbits
Skin / cytology*,  drug effects*
Tensile Strength
Zein / chemistry*,  pharmacology*,  ultrastructure
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 9010-66-6/Zein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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