Document Detail


In vivo application of optogenetics for neural circuit analysis.
MedLine Citation:
PMID:  22896801     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Optogenetics combines optical and genetic methods to rapidly and reversibly control neural activities or other cellular functions. Using genetic methods, specific cells or anatomical pathways can be sensitized to light through exogenous expression of microbial light activated opsin proteins. Using optical methods, opsin expressing cells can be rapidly and reversibly controlled by pulses of light of specific wavelength. With the high spatial temporal precision, optogenetic tools have enabled new ways to probe the causal role of specific cells in neural computation and behavior. Here, we overview the current state of the technology, and provide a brief introduction to the practical considerations in applying optogenetics in vivo to analyze neural circuit functions.
Authors:
Xue Han
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2012-07-16
Journal Detail:
Title:  ACS chemical neuroscience     Volume:  3     ISSN:  1948-7193     ISO Abbreviation:  ACS Chem Neurosci     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-16     Completed Date:  2013-04-10     Revised Date:  2013-08-18    
Medline Journal Info:
Nlm Unique ID:  101525337     Medline TA:  ACS Chem Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  577-84     Citation Subset:  IM    
Affiliation:
Biomedical Engineering Department, Boston University , Boston, Massachusetts, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Electrodes
Electrophysiological Phenomena
Genetic Vectors
Humans
Ion Channels / physiology,  radiation effects
Light
Molecular Probes*
Nerve Net / physiology*,  radiation effects
Neurons / physiology
Opsins / diagnostic use
Photic Stimulation
Rhodopsin / genetics,  physiology
Signal Transduction / drug effects,  physiology
Viruses / genetics
Grant Support
ID/Acronym/Agency:
1R01NS081716/NS/NINDS NIH HHS; 5R00MH085944/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Ion Channels; 0/Molecular Probes; 0/Opsins; 0/channelrhodopsin 2, human; 9009-81-8/Rhodopsin
Comments/Corrections

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