Document Detail


In vivo anergized CD4+ T cells have defective expression and function of the activating protein-1 transcription factor.
MedLine Citation:
PMID:  9834073     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transcription factor activating protein-1 (AP-1) contributes significantly to the regulation of IL-2 gene expression during T cell activation and has been suggested to play a unique role in T cell anergy in vitro. In this study we have used the superantigen staphylococcal enterotoxin A to investigate the regulation of AP-1 in T cell anergy in vivo. Repeated injections of staphylococcal enterotoxin A induce a state of anergy in CD4+ T cells, characterized by reduced expression of IL-2 at mRNA and protein levels. The perturbed IL-2 response in anergic T cells correlated with reduced DNA binding activity of the transcription factors AP-1 and Fos/Jun-containing NF-AT. Using AP-1-luciferase reporter transgenic mice, we now demonstrate the lack of AP-1-dependent transcription. AP-1 activity is controlled by synthesis of its subunits Fos and Jun and by posttranslational phosphorylations. Analysis of Fos and Jun protein levels revealed no major differences in the expression of Jun proteins, but a marked decrease in c-Fos in anergic T cells. Experiments in transgenic mice overexpressing c-Fos (H2-c-fos) showed reconstituted AP-1 DNA binding. In contrast, the AP-1-driven transcription and IL-2 production remained suppressed. The Jun N-terminal kinase is known to play a critical role in regulating AP-1 trans-activation. Analyses of Jun N-terminal kinase demonstrated normal protein amounts, but reduced enzymatic activity, in anergic compared with activated CD4+ T cells. This suggests that in vivo anergized T cells have defects in the AP-1 pathway due to both reduced protein expression and perturbed posttranslational modifications.
Authors:
A Sundstedt; M Dohlsten
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  161     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1998-12-21     Completed Date:  1998-12-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5930-6     Citation Subset:  AIM; IM    
Affiliation:
Pharmacia & Upjohn, Lund Research Center, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Animals
CD4-Positive T-Lymphocytes / enzymology,  immunology*,  metabolism*
Calcium-Calmodulin-Dependent Protein Kinases / deficiency,  metabolism
Clonal Anergy* / genetics
DNA-Binding Proteins / metabolism
Enzyme Activation / genetics,  immunology
H-2 Antigens / genetics
Injections, Intravenous
JNK Mitogen-Activated Protein Kinases
Mice
Mice, Inbred C57BL
Mice, Transgenic
Mitogen-Activated Protein Kinases*
Protein Binding / genetics,  immunology
Proto-Oncogene Proteins c-fos / biosynthesis,  deficiency,  genetics
Superantigens / administration & dosage,  pharmacology
Transcription Factor AP-1 / deficiency*,  genetics*,  physiology
Transcription, Genetic / immunology
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/H-2 Antigens; 0/Proto-Oncogene Proteins c-fos; 0/Superantigens; 0/Transcription Factor AP-1; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases

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