Document Detail

In vivo analysis using variants of zebrafish BMPR-IA: range of action and involvement of BMP in ectoderm patterning.
MedLine Citation:
PMID:  9834197     Owner:  NLM     Status:  MEDLINE    
It has been an intriguing problem whether the polypeptide growth factors belonging to the transforming growth factor-beta (TGF-beta) superfamily function as direct and long-range signaling molecules in pattern formation of the early embryo. In this study, we examined the mechanism of signal propagation of bone morphogenetic protein (BMP) in the ectodermal patterning of zebrafish embryos, in which BMP functions as an epidermal inducer and a neural inhibitor. To estimate the effective range of zbmp-2, we first performed whole-mount in situ hybridization analysis. The zbmp-2-expressing domain and the neuroectoderm, marked by otx-2 expression, were complementary, suggesting that BMP has a short-range effect in vivo. Moreover, mosaic experiments using a constitutively active form of a zebrafish BMP type I receptor (CA-BRIA) demonstrated that the cell-fate conversion, revealed by ectopic expression of gata-3 and repression of otx-2, occurred in a cell-autonomous manner, denying the involvement of the relay mechanism. We also found that zbmp-2 was induced cell autonomously within the transplanted cells in the host ectoderm, suggesting that BMP cannot influence even the neighboring cells. This result is consistent with the observation that there is no gap between the expression domains of zbmp-2 and otx-2. Taken together, we propose that, in ectodermal patterning, BMP exerts a direct and cell-autonomous effect to fate uncommitted ectodermal cells to become epidermis.
M Nikaido; M Tada; H Takeda; A Kuroiwa; N Ueno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  126     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-22     Completed Date:  1999-02-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  181-90     Citation Subset:  IM    
Department of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Body Patterning / physiology*
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein Receptors, Type I
Bone Morphogenetic Proteins / genetics,  metabolism*
Cloning, Molecular
Ectoderm / physiology*
Embryo, Nonmammalian
Embryonic Induction / physiology
Homeodomain Proteins*
Molecular Sequence Data
Nerve Tissue Proteins / genetics,  metabolism
Otx Transcription Factors
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Receptors, Growth Factor / genetics,  metabolism*
Recombinant Proteins / genetics,  metabolism
Sequence Homology, Amino Acid
Signal Transduction
Trans-Activators / genetics,  metabolism
Transforming Growth Factor beta*
Xenopus laevis / genetics
Zebrafish / embryology*,  genetics
Zebrafish Proteins
Reg. No./Substance:
0/Bone Morphogenetic Protein 2; 0/Bone Morphogenetic Proteins; 0/Homeodomain Proteins; 0/Nerve Tissue Proteins; 0/Otx Transcription Factors; 0/Otx2 protein, zebrafish; 0/Receptors, Growth Factor; 0/Recombinant Proteins; 0/Trans-Activators; 0/Transforming Growth Factor beta; 0/Zebrafish Proteins; EC Kinases; EC Morphogenetic Protein Receptors, Type I

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