Document Detail

In vivo analysis of a new R5 tropic SHIV generated from the highly pathogenic SHIV-KS661, a derivative of SHIV-89.6.
MedLine Citation:
PMID:  20102777     Owner:  NLM     Status:  MEDLINE    
Although X4 tropic SHIVs have been studied extensively, they show distinct infection phenotypes from those of R5 tropic viruses, which play an important role in HIV-1 transmission and pathogenesis. To augment the variety of R5 tropic SHIVs, we generated a new R5 tropic SHIV from the highly pathogenic X4 tropic SHIV-KS661, a derivative of SHIV-89.6. Based on consensus amino acid alignment analyses of subtype B R5 tropic HIV-1, five amino acid substitutions in the third variable region successfully changed the secondary receptor preference from X4 to R5. Improvements in viral replication were observed in infected rhesus macaques after two passages, and reisolated virus was designated SHIV-MK38. SHIV-MK38 maintained R5 tropism through in vivo passages and showed robust replication in infected monkeys. Our study clearly demonstrates that a minimal number of amino acid substitutions in the V3 region can alter secondary receptor preference and increase the variety of R5 tropic SHIVs.
Kenta Matsuda; Katsuhisa Inaba; Yoshinori Fukazawa; Megumi Matsuyama; Kentaro Ibuki; Mariko Horiike; Naoki Saito; Masanori Hayami; Tatsuhiko Igarashi; Tomoyuki Miura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-27
Journal Detail:
Title:  Virology     Volume:  399     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-01     Completed Date:  2010-03-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  134-43     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Laboratory of Primate Model, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoinkawaramachi, Sakyo-ku, Kyoto 606-8507, Japan.
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MeSH Terms
Amino Acid Sequence
CD4-Positive T-Lymphocytes
Macaca mulatta / virology
Mutagenesis, Site-Directed
Sequence Alignment
Simian Acquired Immunodeficiency Syndrome / virology*
Simian immunodeficiency virus / genetics,  isolation & purification,  pathogenicity*,  physiology
Species Specificity
Viral Load
Viral Proteins / genetics
Virus Replication / physiology
Reg. No./Substance:
0/Viral Proteins

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