| In vivo analysis of cell division, cell growth, and differentiation at the shoot apical meristem in Arabidopsis. | |
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MedLine Citation:
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PMID: 14671026 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aerial parts of the plant are generated by groups of rapidly dividing cells called shoot apical meristems. To analyze cell behavior in these structures, we developed a technique to visualize living shoot apical meristems using the confocal microscope. This method, combined with green fluorescent protein marker lines and vital stains, allows us to follow the dynamics of cell proliferation, cell expansion, and cell differentiation at the shoot apex. Using this approach, the effects of several mitotic drugs on meristem development were studied. Oryzalin (depolymerizing microtubules) very rapidly caused cell division arrest. Nevertheless, both cell expansion and cell differentiation proceeded in the treated meristems. Interestingly, DNA synthesis was not blocked, and the meristematic cells went through several rounds of endoreduplication in the presence of the drug. We next treated the meristems with two inhibitors of DNA synthesis, aphidicolin and hydroxyurea. In this case, cell growth and, later, cell differentiation were inhibited, suggesting an important role for DNA synthesis in growth and patterning. |
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Authors:
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Olivier Grandjean; Teva Vernoux; Patrick Laufs; Katia Belcram; Yuki Mizukami; Jan Traas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-12-11 |
Journal Detail:
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Title: The Plant cell Volume: 16 ISSN: 1040-4651 ISO Abbreviation: Plant Cell Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-01-05 Completed Date: 2004-04-28 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
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Languages: eng Pagination: 74-87 Citation Subset: IM |
Affiliation:
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Institut National de la Recherche Agronomique, Laboratoire Commun de Cytologie, Institut Jean-Pierre Bourgin, 78026 Versailles Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aphidicolin
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pharmacology Arabidopsis / cytology, genetics, growth & development* Cell Cycle / drug effects, physiology* Cell Differentiation / drug effects, physiology* DNA, Plant / drug effects, genetics Dinitrobenzenes / pharmacology Hydroxyurea / pharmacology Meristem / cytology, drug effects, growth & development* Microscopy, Confocal Nucleic Acid Synthesis Inhibitors / pharmacology Plant Shoots / cytology, growth & development Sulfanilamides* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Plant; 0/Dinitrobenzenes; 0/Nucleic Acid Synthesis Inhibitors; 0/Sulfanilamides; 127-07-1/Hydroxyurea; 19044-88-3/oryzalin; 38966-21-1/Aphidicolin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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