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In vivo analysis of cadherin function in the mouse intestinal epithelium: essential roles in adhesion, maintenance of differentiation, and regulation of programmed cell death.
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MedLine Citation:
PMID:  7721948     Owner:  NLM     Status:  MEDLINE    
A model system is described for defining the physiologic functions of mammalian cadherins in vivo. 129/Sv embryonic stem (ES) cells, stably transfected with a dominant negative N-cadherin mutant (NCAD delta) under the control of a promoter that only functions in postmitotic enterocytes during their rapid, orderly, and continuous migration up small intestinal villi, were introduced into normal C57B1/6 (B6) blastocysts. In adult B6<->129/Sv chimeric mice, each villus receives the cellular output of several surrounding monoclonal crypts. A polyclonal villus located at the boundary of 129/Sv- and B6-derived intestinal epithelium contains vertical coherent bands of NCAD delta-producing enterocytes plus adjacent bands of normal B6-derived enterocytes. A comparison of the biological properties of these cell populations established that NCAD delta disrupts cell-cell and cell-matrix contacts, increases the rate of migration of enterocytes along the crypt-villus axis, results in a loss of their differentiated polarized phenotype, and produces precocious entry into a death program. These data indicate that enterocytic cadherins are critical cell survival factors that actively maintain intestinal epithelial function in vivo.
M L Hermiston; J I Gordon
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  129     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-05-22     Completed Date:  1995-05-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  489-506     Citation Subset:  IM    
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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MeSH Terms
Actins / analysis
Apoptosis / physiology*
Base Sequence
Cadherins / analysis,  physiology*
Cell Adhesion / physiology*
Cell Communication / physiology
Cell Differentiation / physiology*
Cell Movement
Epithelial Cells
Epithelium / chemistry
Intestines / chemistry,  cytology*,  microbiology
Mice, Inbred C57BL
Mice, Transgenic
Microvilli / physiology
Models, Biological
Molecular Sequence Data
Sequence Deletion / physiology
Grant Support
Reg. No./Substance:
0/Actins; 0/Cadherins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 2 Month: 4 Year: 1995
Volume: 129 Issue: 2
First Page: 489 Last Page: 506
ID: 2199905
Publisher Id: 95238544
PubMed Id: 7721948

In vivo analysis of cadherin function in the mouse intestinal epithelium: essential roles in adhesion, maintenance of differentiation, and regulation of programmed cell death

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