Document Detail


In vivo administration of recombinant TNF-alpha promotes bone resorption in mice.
MedLine Citation:
PMID:  12828665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In vitro studies demonstrated that proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha) modulates bone metabolism. OBJECTIVE: The aim of our study was to confirm the ability of TNF-alpha to induce osteoclastogenesis and bone resorption in an in vivo experiment, with the use of calvarial model in mice. MATERIALS AND METHODS: Twenty C57-Black mice were divided into four groups with five animals in each. The first group was infused subcutaneously on their back with recombinant mouse (rm) TNF-alpha via osmotic minipumps for 3 d, the second group was similarly infused with phosphate-buffered saline (PBS), the third group was infused with rmTNF-alpha to the region above the parietal bone and the fourth group with PBS in the same manner. Number of osteoclasts on parietal bone was determined morphometrically. Serum calcium and phosphates were monitored colorimetrically. RESULTS: Serum calcium level and number of osteoclasts on parietal bone were significantly greater after infusion of rmTNF-alpha above the parietal bone, whereas after subcutaneous delivery these parameters were similar to the control group. CONCLUSION: We are concluding that TNF-alpha has the ability to change the bone metabolism in a paracrine manner only.
Authors:
Rok Gaspersic; Draga Stiblar-Martincic; Josko Osredkar; Uros Skaleric
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of periodontal research     Volume:  38     ISSN:  0022-3484     ISO Abbreviation:  J. Periodont. Res.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-06-27     Completed Date:  2003-10-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0055107     Medline TA:  J Periodontal Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  446-8     Citation Subset:  D; IM    
Affiliation:
Department of Oral Medicine and Periodontology, Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana and Clinical Institute of Clinical Chemistry and Biochemistry, Clinical Centre, Ljubljana, Slovenia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / drug effects
Bone Resorption / chemically induced*,  pathology
Calcium / blood
Cell Count
Image Processing, Computer-Assisted
Injections, Subcutaneous
Male
Mice
Mice, Inbred C57BL
Osteoclasts / drug effects,  pathology
Paracrine Communication
Parietal Bone / drug effects,  pathology
Phosphates / blood
Placebos
Recombinant Proteins
Tumor Necrosis Factor-alpha / administration & dosage,  adverse effects*
Chemical
Reg. No./Substance:
0/Phosphates; 0/Placebos; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 7440-70-2/Calcium

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