Document Detail

In vivo acidosis reduces extracellular concentrations of taurine and glutamate in the rat hippocampus.
MedLine Citation:
PMID:  7913139     Owner:  NLM     Status:  MEDLINE    
Microdialysis-perfusion of the urethane-anesthetized rat hippocampus was performed to assess the effects of acidosis on extracellular amino acids. Perfusion with Krebs-Ringer bicarbonate buffer at pH 6.9 produced a selective decrease in taurine. A further reduction of pH to 6.4 induced diminished glutamate levels. The Cl-/HCO3- exchange inhibitor 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene (DIDS) did not affect interstitial taurine during perfusion, but there was a rebound increase in taurine levels upon withdrawal of the agent. In contrast, glutamate concentrations were elevated during DIDS administration, and decreased upon reperfusion with standard buffer. The reduction of extracellular taurine and glutamate concentrations caused by low pH was inhibited by DIDS. The results suggest that taurine and glutamate uptake and/or release in vivo is pH-dependent, and that the effects of acidosis possibly are mediated by the Cl-/HCO3- antiporter. The decrease in extracellular glutamate brought about by low pH may have pathophysiologic implications in conditions associated with disturbed pH homeostasis such as cerebral ischemia and spreading depression.
M Puka; A Lehmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  37     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-08-09     Completed Date:  1994-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  641-6     Citation Subset:  IM    
Department of Neurochemistry, Medical Research Centre, Polish Academy of Sciences, Warsaw.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Acidosis / metabolism*
Antiporters / antagonists & inhibitors,  metabolism*
Bicarbonates / metabolism
Brain Ischemia / metabolism*
Chloride-Bicarbonate Antiporters
Chlorides / metabolism
Extracellular Space / metabolism*
Glutamates / metabolism*
Glutamic Acid
Hippocampus / metabolism*
Hydrogen-Ion Concentration
Nerve Tissue Proteins / antagonists & inhibitors,  metabolism*
Osmotic Pressure
Rats, Sprague-Dawley
Sodium-Hydrogen Antiporter / metabolism
Taurine / metabolism*
Reg. No./Substance:
0/Antiporters; 0/Bicarbonates; 0/Chloride-Bicarbonate Antiporters; 0/Chlorides; 0/Glutamates; 0/Nerve Tissue Proteins; 0/Sodium-Hydrogen Antiporter; 107-35-7/Taurine; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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