Document Detail


In vivo acidosis reduces extracellular concentrations of taurine and glutamate in the rat hippocampus.
MedLine Citation:
PMID:  7913139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microdialysis-perfusion of the urethane-anesthetized rat hippocampus was performed to assess the effects of acidosis on extracellular amino acids. Perfusion with Krebs-Ringer bicarbonate buffer at pH 6.9 produced a selective decrease in taurine. A further reduction of pH to 6.4 induced diminished glutamate levels. The Cl-/HCO3- exchange inhibitor 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene (DIDS) did not affect interstitial taurine during perfusion, but there was a rebound increase in taurine levels upon withdrawal of the agent. In contrast, glutamate concentrations were elevated during DIDS administration, and decreased upon reperfusion with standard buffer. The reduction of extracellular taurine and glutamate concentrations caused by low pH was inhibited by DIDS. The results suggest that taurine and glutamate uptake and/or release in vivo is pH-dependent, and that the effects of acidosis possibly are mediated by the Cl-/HCO3- antiporter. The decrease in extracellular glutamate brought about by low pH may have pathophysiologic implications in conditions associated with disturbed pH homeostasis such as cerebral ischemia and spreading depression.
Authors:
M Puka; A Lehmann
Related Documents :
12788509 - Insulin affects synaptosomal gaba and glutamate transport under oxidative stress condit...
9130799 - The glutathione level of retinal müller glial cells is dependent on the high-affinity ...
15263929 - Shape control of anatase tio2 nanoparticles by amino acids in a gel-sol system.
22236139 - Efficacy of cosmetic formulations containing dispersion of liposome with magnesium asco...
8304149 - New thiophene substituted 10-deazaaminopterins: synthesis and biological evaluation.
16879709 - Activity-dependent cleavage of brain glutamic acid decarboxylase 65 by calpain.
5413329 - The role of immunogenicity in the induction of tolerance with conjugates of arsanilic a...
2194199 - Bile salt-fatty acid mixed micelles as nasal absorption promoters of peptides. ii. in v...
16303299 - Effect of oral nitroethane and 2-nitropropanol administration on methane-producing acti...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  37     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-08-09     Completed Date:  1994-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  641-6     Citation Subset:  IM    
Affiliation:
Department of Neurochemistry, Medical Research Centre, Polish Academy of Sciences, Warsaw.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Acidosis / metabolism*
Animals
Antiporters / antagonists & inhibitors,  metabolism*
Bicarbonates / metabolism
Brain Ischemia / metabolism*
Chloride-Bicarbonate Antiporters
Chlorides / metabolism
Extracellular Space / metabolism*
Glutamates / metabolism*
Glutamic Acid
Hippocampus / metabolism*
Hydrogen-Ion Concentration
Male
Microdialysis
Nerve Tissue Proteins / antagonists & inhibitors,  metabolism*
Osmotic Pressure
Perfusion
Rats
Rats, Sprague-Dawley
Sodium-Hydrogen Antiporter / metabolism
Taurine / metabolism*
Chemical
Reg. No./Substance:
0/Antiporters; 0/Bicarbonates; 0/Chloride-Bicarbonate Antiporters; 0/Chlorides; 0/Glutamates; 0/Nerve Tissue Proteins; 0/Sodium-Hydrogen Antiporter; 107-35-7/Taurine; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Expression of p160erbB-3 and p185erbB-2 in prostatic intraepithelial neoplasia and prostatic adenoca...
Next Document:  Elevated levels of soluble ICAM-1 correlate with the development of multiple organ failure in severe...