| In vivo wound healing activity of the methanolic extract and its isolated constituent, gulonic acid gamma-lactone, obtained from Grewia tiliaefolia. | |
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MedLine Citation:
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PMID: 19219758 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Grewia tiliaefolia is a subtropical tree, its stem bark is widely used in traditional Indian medicines to heal chronic wounds, gastric ulcers, burning sensation, itching and other allergic ailments. Bioassay-directed fractionation and chromatography of the methanolic extract of G. tiliaefolia stem bark has resulted in the isolation of gulonic acid gamma-lactone. The methanolic extract and the isolated constituent were studied for their potency on three different cutaneous wound models, VIZ., excision, incision and dead space wounds in Wistar rats. In the excision wound model, healing was assessed by the rate of wound contraction and period of epithelisation. In the incision wound model, the degree of healing was analysed by determining the skin breaking strength. In the dead space wound model, the parameters used to confirm the healing process were weight of granulation tissue, its tensile strength, hydroxyproline content and histological studies. The extract as well as the constituent demonstrated wound healing activity. Topical application of gulonic acid gamma-lactone (0.2% w/w ointment) caused faster epithelialisation with 94.02% wound contraction on day 16 post-wounding, while in control animals the duration of healing was extended up to 22 days with 79.53% wound contraction. The tensile strength of the incision wound was significantly increased (561.12 +/- 5.18 g) compared to the control (327.63 +/- 6.37 g). In the dead space wound model, a significant increase in weight, tensile strength and hydroxyproline content of the granuloma tissue was observed following oral administration of gulonic acid gamma-lactone (60 mg/kg). Histology of the granuloma tissue showed increased collagenation and the absence of monocytes. The wound healing effect was compared with that of the standard skin ointment nitrofurazone. The results of this investigation provide supportive scientific evidence for the medicinal use of G. tiliaefolia for healing of cutaneous wound. |
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Authors:
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B Mohamed Khadeer Ahamed; Venkatarangaiah Krishna; Kumaraswamy H Malleshappa |
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Publication Detail:
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Type: Journal Article Date: 2009-02-13 |
Journal Detail:
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Title: Planta medica Volume: 75 ISSN: 1439-0221 ISO Abbreviation: Planta Med. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-07 Completed Date: 2009-09-15 Revised Date: 2010-05-17 |
Medline Journal Info:
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Nlm Unique ID: 0066751 Medline TA: Planta Med Country: Germany |
Other Details:
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Languages: eng Pagination: 478-82 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Basaveshwara Medical College, Hospital and Research Centre, Rajiv Gandhi University, Chitradurga, Karnataka, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Topical Animals Anti-Infective Agents / administration & dosage, pharmacology Dose-Response Relationship, Drug Female Granulation Tissue / drug effects, pathology Grewia / chemistry* Lactones / administration & dosage, isolation & purification, pharmacology* Male Nitrofurazone / administration & dosage, pharmacology Ointments / pharmacology Plant Bark Plant Extracts / administration & dosage, chemistry, pharmacology* Plant Stems Rats Rats, Wistar Skin / drug effects*, pathology Sugar Acids / administration & dosage, isolation & purification, pharmacology* Tensile Strength / drug effects Wound Healing / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents; 0/Lactones; 0/Ointments; 0/Plant Extracts; 0/Sugar Acids; 20246-33-7/gulonic acid; 59-87-0/Nitrofurazone; 90-80-2/beta-glucono-1,5-lactone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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