Document Detail


In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains.
MedLine Citation:
PMID:  22546689     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The creation of molecular tools able to unravel in vivo spatiotemporal activation of specific cell signaling events during cell migration, differentiation and morphogenesis is of great relevance to developmental cell biology. Here, we describe the generation, validation and applications of two transgenic reporter lines for Wnt/β-catenin signaling, named TCFsiam, and show that they are reliable and sensitive Wnt biosensors for in vivo studies. We demonstrate that these lines sensitively detect Wnt/β-catenin pathway activity in several cellular contexts, from sensory organs to cardiac valve patterning. We provide evidence that Wnt/β-catenin activity is involved in the formation and maintenance of the zebrafish CNS blood vessel network, on which sox10 neural crest-derived cells migrate and proliferate. We finally show that these transgenic lines allow for screening of Wnt signaling modifying compounds, tissue regeneration assessment as well as evaluation of potential Wnt/β-catenin genetic modulators.
Authors:
Enrico Moro; Gunes Ozhan-Kizil; Alessandro Mongera; Dimitris Beis; Claudia Wierzbicki; Rodrigo M Young; Despina Bournele; Alice Domenichini; Leonardo E Valdivia; Lawrence Lum; Chuo Chen; James F Amatruda; Natascia Tiso; Gilbert Weidinger; Francesco Argenton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-21
Journal Detail:
Title:  Developmental biology     Volume:  366     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-21     Completed Date:  2013-07-05     Revised Date:  2014-02-24    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  327-40     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Biosensing Techniques
Cell Differentiation
Cell Movement
Neurons / cytology,  physiology
Wnt Signaling Pathway*
Zebrafish / embryology,  physiology*
Grant Support
ID/Acronym/Agency:
R01 CA135731/CA/NCI NIH HHS

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