| In vivo matrix metalloproteinase-7 substrates identified in the left ventricle post-myocardial infarction using proteomics. | |
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MedLine Citation:
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PMID: 20232908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matrix metalloproteinase-7 (MMP-7) deletion has been shown to improve survival after myocardial infarction (MI). MMP-7 has a large array of in vitro substrates, but in vivo substrates for MMP-7 following MI have not been fully identified. Accordingly, we evaluated the infarct regions of wild-type (WT; n = 12) and MMP-7 null (null; n = 10) mice using a proteomic strategy. Seven days post-MI, infarct regions of the left ventricles were excised, homogenized, and protein extracts were analyzed by two-dimensional gel electrophoresis and mass spectrometry. Of 13 spots that showed intensity differences between WT and null, the intensities of eight spots were higher and those of five spots were lower in the null group (p < 0.05). Fibronectin and tenascin-C, known in vitro substrates of MMP-7, were identified in spots that showed lower intensity in the null. Immunoblotting and in vitro cleavage assays confirmed reduced fibronectin and tenascin-C fragment generation in the null, and this effect was restored by exogenous administration of MMP-7. Lower levels of full-length peroxiredoxin-1 and -2 and higher levels of the full-length peroxiredoxin-3 were detected in the null group, suggesting MMP-7 deletion may also indirectly regulate protein levels through nonenzymatic mechanisms. In conclusion, this is the first study to identify fibronectin and tenascin-C as in vivo MMP-7 substrates in the infarcted left ventricle using a proteomic approach. |
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Authors:
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Ying Ann Chiao; Rogelio Zamilpa; Elizabeth F Lopez; Qiuxia Dai; Gladys P Escobar; Kevin Hakala; Susan T Weintraub; Merry L Lindsey |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of proteome research Volume: 9 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-07 Completed Date: 2010-08-12 Revised Date: 2013-02-18 |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 2649-57 Citation Subset: IM |
Affiliation:
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Department of Medicine, Division of Cardiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electrophoresis, Gel, Two-Dimensional Fibronectins / analysis, metabolism Heart Ventricles / anatomy & histology, enzymology*, pathology Immunoblotting Male Mass Spectrometry Matrix Metalloproteinase 7 / genetics, metabolism* Mice Mice, Inbred C57BL Mice, Transgenic Myocardial Infarction / enzymology* Myocardium / enzymology* Peroxiredoxins / analysis, metabolism Proteomics / methods* Tenascin / analysis, metabolism Ventricular Remodeling / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HL75360/HL/NHLBI NIH HHS; R01 HL075360/HL/NHLBI NIH HHS; R01 HL075360-06/HL/NHLBI NIH HHS; R01 HL075360-06S1/HL/NHLBI NIH HHS; R01 HL075360-09/HL/NHLBI NIH HHS; T32 HL07446/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 0/Tenascin; EC 1.11.1.15/Peroxiredoxins; EC 3.4.24.23/Matrix Metalloproteinase 7 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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