Document Detail


In vivo [Fe-S] cluster acquisition by IscR and NsrR, two stress regulators in Escherichia coli.
MedLine Citation:
PMID:  23320508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The multi-proteins Isc and Suf systems catalyse the biogenesis of [Fe-S] proteins. Here we investigate how NsrR and IscR, transcriptional regulators that sense NO and [Fe-S] homeostasis, acquire their [Fe-S] clusters under both normal and iron limitation conditions. Clusters directed at the apo-NsrR and apo-IscR proteins are built on either of the two scaffolds, IscU or SufB. However, differences arise in [Fe-S] delivery steps. In the case of NsrR, scaffolds deliver clusters to either one of the two ATCs, IscA and SufA, and, subsequently, to the 'non-Isc non-Suf' ATC, ErpA. Nevertheless, a high level of SufA can bypass the requirement for ErpA. In the case of IscR, several routes occur. One does not include assistance of any ATC. Others implicate ATCs IscA or ErpA, but, surprisingly, SufA was totally absent from any IscR maturation pathways. Both IscR and NsrR have the intrinsic capacity to sense iron limitation. However, NsrR appeared to be efficiently matured by Isc and Suf, thereby preventing NsrR to act as a physiologically relevant iron sensor. This work emphasizes that different maturation pathways arise as a function of the apo-target considered, possibly in relation with the type of cluster, [2Fe-2S] versus [4Fe-4S], it binds.
Authors:
Daniel Vinella; Laurent Loiseau; Sandrine Ollagnier de Choudens; Marc Fontecave; Frédéric Barras
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-16
Journal Detail:
Title:  Molecular microbiology     Volume:  87     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-25     Completed Date:  2013-07-05     Revised Date:  2013-10-31    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  493-508     Citation Subset:  IM    
Copyright Information:
© 2013 Blackwell Publishing Ltd.
Affiliation:
Laboratoire de Chimie Bactérienne, UMR 7283 Aix-Marseille Université-CNRS, Institut de Microbiologie de la Méditerranée, 31 Chemin Joseph Aiguier, 13009 Marseille, France.
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MeSH Terms
Descriptor/Qualifier:
DNA-Binding Proteins / metabolism*
Escherichia coli / genetics*,  metabolism*
Escherichia coli Proteins / metabolism*
Gene Expression Regulation, Bacterial*
Iron / metabolism*
Protein Processing, Post-Translational
Sulfur / metabolism*
Transcription Factors / metabolism*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Escherichia coli Proteins; 0/IscR protein, E coli; 0/NsrR protein, E coli; 0/Transcription Factors; 7439-89-6/Iron; 7704-34-9/Sulfur

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