Document Detail


In vivo EPR studies of the metabolic fate of nitrosobenzene in the mouse.
MedLine Citation:
PMID:  8121274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the first demonstration of EPR spectroscopy to study free radical reactions in live mice and excised muscle tissue resulting from the metabolism of nitrosobenzene. A broad three-line EPR spectrum (aN = 11.6 G) appeared in the buttocks region of a mouse place in an L-band loop gap resonator after intramuscular or intraperitoneal injection of 0.2 mmol/kg nitrosobenzene. The signal intensity reached a maximum at 20 to 30 min and remained constant well beyond 2 h. If muscle tissue was dosed with nitrosobenzene and excised within 5 min, a similar three-line X-band EPR spectrum was obtained which was preceded by the rapid growth and subsequent decay of an EPR spectrum identical with that of the phenylhydronitroxide radical, which was presumably generated from reactions between nitrosobenzene and reducing agents in the blood or tissue such as NADH or ascorbic acid. A model system containing nitrosobenzene and unsaturated fatty acids (olive oil or animal fat) yielded an identical three-line spectrum resulting from radical adducts of nitrosobenzene across the double bond. Overall, these results suggest that the most probable mechanism in vivo was nitrosobenzene covalently adding ("binding") to polyunsaturated fatty acid clusters in fat or membranes.
Authors:
H Fujii; B Zhao; J Koscielniak; L J Berliner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  31     ISSN:  0740-3194     ISO Abbreviation:  Magn Reson Med     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-04-04     Completed Date:  1994-04-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  77-80     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Ohio State University, Columbus 43210.
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MeSH Terms
Descriptor/Qualifier:
Animals
Electron Spin Resonance Spectroscopy*
Male
Mice
Mice, Inbred BALB C
Muscles / metabolism
Nitroso Compounds / pharmacokinetics*
Grant Support
ID/Acronym/Agency:
RR03126/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Nitroso Compounds; 586-96-9/nitrosobenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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