Document Detail

In-vivo Candida biofilms in scanning electron microscopy.
MedLine Citation:
PMID:  19888801     Owner:  NLM     Status:  MEDLINE    
Candida biofilms on indwelling devices are an increasing problem in patients treated at intensive care units. The goal of this study was to examine the occurrence and frequency of these biofilms. A total of 172 catheters were collected from 105 male and 67 female patients (the age range of both patient groups was from 3 weeks to 98 years old). The catheters were incubated on blood agar plates and the resulting yeast colonies were subsequently identified. Furthermore, pieces of catheters were fixed, dried and sputter coated with gold for investigation with scanning electron microscopy (SEM). Yeasts were recovered from significantly more catheters obtained from men than from women (chi(2): n = 67; P < 0.01). In SEM, 56.4% catheters turned out to be positive for biofilm formation. Again catheters from male patients were statistically significant (chi(2): n = 40; P < 0.01) more often positive than those from women. Candida albicans (71.1%) was the most common species isolated from the catheters, followed by C. glabrata (10.3%), C. parapsilosis (8.2%) and C. tropicalis (5.2%). Based on the results of this investigation, the epidemiology of Candida biofilms on indwelling devices seems to be a promising target for future investigations.
Astrid Helga Paulitsch; Birgit Willinger; Benedikt Zsalatz; Edith Stabentheiner; Egon Marth; Walter Buzina
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical mycology     Volume:  47     ISSN:  1460-2709     ISO Abbreviation:  Med. Mycol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-02-12     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  9815835     Medline TA:  Med Mycol     Country:  England    
Other Details:
Languages:  eng     Pagination:  690-6     Citation Subset:  IM    
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MeSH Terms
Age Factors
Aged, 80 and over
Candida / classification,  isolation & purification*
Candidiasis / epidemiology,  microbiology
Catheters, Indwelling
Chi-Square Distribution
Child, Preschool
Cross-Sectional Studies
Infant, Newborn
Microscopy, Electron, Scanning / methods*
Middle Aged
Sex Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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