Document Detail


"In vivo" (35S)methionine interaction with rat liver tRNA.
MedLine Citation:
PMID:  2775265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As part of a study to characterize the methionine role in tumorigenesis, we report that methionine sulfur interacts with rat liver tRNA "in vivo" (35S) radioactivity remained associated to the nucleic acid after a number of treatments, including tRNA deacylation. Similar data were obtained after administration of (methyl-3H) methionine, while no comparable tRNA labelling was detected when the aminoacid labelled in the aliphatic chain was given. Hplc analysis of (35S) tRNA enzymic hydrolysate showed two unidentified UV-absorbing radioactive peaks. NMR spectra of these two peaks did not reveal any thiomethyl group.
Authors:
D Kanduc; M R Rossiello; T Ranieri; E Quagliariello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  163     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-10-06     Completed Date:  1989-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
Affiliation:
Dipartimento di Biochimica e Biologia Moleculare, Facoltá di Scienze, Universitá di Bari, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA / metabolism
Liver / physiology*
Magnetic Resonance Spectroscopy
Methionine / metabolism*
RNA, Transfer / metabolism*
Rats
Spectrophotometry, Ultraviolet
Sulfur*
Chemical
Reg. No./Substance:
63-68-3/Methionine; 7704-34-9/Sulfur; 9007-49-2/DNA; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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