Document Detail


In vivo 31P nuclear magnetic resonance saturation transfer measurements of phosphate exchange reactions in the yeast Saccharomyces cerevisiae.
MedLine Citation:
PMID:  3905437     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
31P saturation transfer techniques have been used to measure phosphate kinetics in the yeast Saccharomyces cerevisiae. The phosphate consumption rate observed in acetate grown mid-log cells was combined with measurements of O2 consumption to yield P/O ratios of 2.2 and 2.9, for cells respiring on glucose and ethanol, respectively. However, no phosphate consumption activity was observed in saturation transfer experiments on anaerobic glucose fed cells. The phosphate consumption rates measured by saturation transfer in cells respiring on glucose and ethanol was attributed to the unidirectional rates of mitochondrial ATP synthesis.
Authors:
S L Campbell; K A Jones; R G Shulman
Related Documents :
12435087 - Extracellular ph affects inflammatory cell production of superoxide and nitric oxide.
2163307 - Establishment of a pancreatic beta cell line that retains glucose-inducible insulin sec...
15047617 - Interaction of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (pfk-2/fbpase-2) wi...
17591847 - Paradoxical growth effect of caspofungin observed on biofilms and planktonic cells of f...
16936717 - Soma-germline interactions coordinate homeostasis and growth in the drosophila gonad.
6491307 - A colorimetric assay for serial determination of adenosine deaminase activity in small ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  193     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-21     Completed Date:  1986-01-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  189-93     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Aerobiosis
Anaerobiosis
Energy Transfer
Glucose / metabolism
Kinetics
Magnetic Resonance Spectroscopy
Mitochondria / metabolism
Models, Chemical
Phosphates / metabolism*
Saccharomyces cerevisiae / metabolism*
Grant Support
ID/Acronym/Agency:
AM 27121/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Phosphates; 50-99-7/Glucose; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Enhancement of growth factor-induced DNA synthesis by colon tumor-promoting bile acids in Swiss 3T3 ...
Next Document:  Energetics of tetracycline efflux system encoded by Tn10 in Escherichia coli.