| In vivo 13C NMR determines metabolic fluxes and steady state in linseed embryos. | |
| | |
MedLine Citation:
|
PMID: 17540418 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The dynamics of developing linseed embryo metabolism was investigated using (13)C-labelling experiments where the real-time kinetics of label incorporation into metabolites was monitored in situ using in vivo NMR. The approach took advantage of the occurrence in this plant tissue of large metabolite pools - such as sucrose or lipids - to provide direct and quantitative measurement of the evolution of the labelling state within central metabolism. As a pre-requisite for the use of steady state flux measurements it was shown that isotopic steady state was reached within 3 h at the level of central intermediates whereas it took a further 6h for the sucrose pool. Complete isotopic and metabolic steady state took 18 h to be reached. The data collected during the transient state where label was equilibrated but the metabolic steady state was incomplete, enabled the rates of lipid and sucrose synthesis to be measured in situ on the same sample. This approach is suitable to get a direct assessment of metabolic time-scales within living plant tissues and provides a valuable complement to steady state flux determinations. |
| | |
Authors:
|
Stéphanie Troufflard; Albrecht Roscher; Brigitte Thomasset; Jean-Noël Barbotin; Stephen Rawsthorne; Jean-Charles Portais |
Related Documents
:
|
20066008 - Atf4 regulates lipid metabolism and thermogenesis. 15314028 - A novel nuclear receptor/coregulator complex controls c. elegans lipid metabolism, larv... 1658998 - Lithium and the phosphoinositide cycle: an example of uncompetitive inhibition and its ... 14197878 - Some biochemical changes in the guinea pig during infection with coxiella burnetii. 7184948 - Toxicology updates. xylene. 4723798 - Acetylcholine release from guinea-pig ileum by parasympathetic ganglion stimulants and ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-30 |
Journal Detail:
|
Title: Phytochemistry Volume: 68 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2007 Aug-Sep |
Date Detail:
|
Created Date: 2007-08-13 Completed Date: 2007-11-05 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
|
Languages: eng Pagination: 2341-50 Citation Subset: IM |
Affiliation:
|
Génie Enzymatique et Cellulaire, UMR-CNRS 6022, Université de Picardie Jules Verne, 33 Rue St-Leu, 80039 Amiens, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carbon Isotopes Flax / embryology*, metabolism Kinetics Lipids / biosynthesis, chemistry Nuclear Magnetic Resonance, Biomolecular / methods Seeds / metabolism* Sucrose / chemistry, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Carbon Isotopes; 0/Lipids; 57-50-1/Sucrose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: An unusual C6-C6" linked flavonoid from Miconia cabucu (Melastomataceae).
Next Document: Isoflavanones from Uraria picta and their antimicrobial activity.