Document Detail


In vitro whole-blood analysis of cellular immunity in patients with active coccidioidomycosis by using the antigen preparation T27K.
MedLine Citation:
PMID:  12204956     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Measurement of cellular immunity in human coccidioidomycosis has important diagnostic and prognostic implications. The coccidioidin skin test has been the standard for the measurement of this, but it is not available in the United States. We examined the utility of measuring surface expression of CD69 on T lymphocytes in whole blood incubated with the coccidioidal antigen preparation T27K as an alternative to the skin test. Seventy donors with active coccidioidomycosis were studied. The mean fluorescent intensity (MFI) of CD69 expression on CD3 lymphocytes in response to T27K was 28.61 +/- 1.77, significantly greater than the control response of 11.45 +/- 0.78 (P < 0.001). The MFI CD69 response to T27K above that for the control (MFI CD69 above control) was 6.35 +/- 2.18 for seven subjects with disseminated coccidioidomycosis who were studied within 5 months of diagnosis. This was significantly below the value of 20.17 +/- 3.17 for 18 subjects with pulmonary coccidioidomycosis studied within 5 months of diagnosis and the value of 19.58 +/- 2.91 for 27 subjects with disseminated coccidioidomycosis studied after 5 months of diagnosis (for both, P < 0.05). There was an inverse correlation between coccidioidal clinical score and MFI CD69 above control for all 34 subjects with disseminated coccidioidomycosis (r = 0.362; P = 0.036) but not for the 36 subjects with pulmonary disease (r < 0.001; P = 0.993). Among 30 subjects for whom data were available, there was a highly significant association between the MFI CD69 above control and the supernatant concentrations of gamma interferon, interleukin-2 (IL-2), and tumor necrosis factor alpha (for all, P < 0.001), but not for IL-4, IL-5, or IL-10. These data indicate that in vitro assessment of CD69 expression on T lymphocytes by using T27K may be a useful measure of cellular immune response among subjects with active coccidioidomycosis.
Authors:
Neil M Ampel; Larissa A Kramer; Lijin Li; Deborah S Carroll; Kathleen M Kerekes; Suzanne M Johnson; Demosthenes Pappagianis
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Clinical and diagnostic laboratory immunology     Volume:  9     ISSN:  1071-412X     ISO Abbreviation:  Clin. Diagn. Lab. Immunol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-02     Completed Date:  2003-02-11     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  9421292     Medline TA:  Clin Diagn Lab Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1039-43     Citation Subset:  IM    
Affiliation:
Medicine and Primary Care Service, Southern Arizona Veterans Affairs Health Care System, Tucson, Arizona 85723, USA. nampel@u.arizona.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Antigens, CD / analysis
Antigens, Differentiation, T-Lymphocyte / analysis
Antigens, Fungal / diagnostic use
Coccidioidomycosis / diagnosis*,  immunology*
Cytokines / biosynthesis
Female
Humans
Immunity, Cellular*
Lectins, C-Type
Male
Middle Aged
T-Lymphocytes / chemistry,  immunology*,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, T-Lymphocyte; 0/Antigens, Fungal; 0/CD69 antigen; 0/Cytokines; 0/Lectins, C-Type
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