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In-vitro and In-vivo Binding Activity of Chicken Egg Yolk Immunoglobulin Y (IgY) Against Gliadin in Food Matrix.
MedLine Citation:
PMID:  22400905     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chicken egg yolk immunoglobulin Y (IgY) is a promising alternative for prevention of enteric gliadin absorption, the predisposing factor of celiac disease (CD). IgY antibody was produced from egg yolk of Single Comb White Leghorn chickens during the immunization period for the development of an oral immunotherapeutic agent. Here, we report the potential use of spray dried IgY antibody formulation using sugar protectants (mannitol, sorbitol or microcrystalline cellulose powder (MCCP)). The long term stability of the spray dried egg yolk powder formulated with 37.5% mannitol (EYP-M) preserved IgY antibody activity at 99.9%, which was significantly higher than with other protectants (p < 0.05). In a dissolution test, the EYP-M shows 82.4% IgY activity after 2 h in simulated gastric fluid (SGF). A competitive ELISA at 50% inhibition (IC50) shows that 1.6 mg/mL EYP-M bound to 7.6 mg/mL and 10.5 mg/mL gliadin in SGF without and with food matrix conditions, respectively, whereas in simulated intestinal fluid the formulation bound to 10 mg/mL gliadin, regardless of a food matrix. In-vivo study; BALB/c mice fed with EYP-M and gliadin at a ratio of 1:5 (w/w), demonstrated that gliadin absorption in the gastrointestinal tract was minimal at < 1%. Thus, EYP-M containing IgY antibody may be used in CD patients to eliminate the effects of ingested toxic gliadin.
Authors:
Naiyana Gujral; R Löbenberg; Mavanur R Suresh; Hoon H Sunwoo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-8
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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