Document Detail

In vitro testing for carcinogens and mutagens.
MedLine Citation:
PMID:  3306977     Owner:  NLM     Status:  MEDLINE    
A large number of in vitro assays for chemical carcinogenicity and mutagenicity have been developed. They have been validated to various extents with the use of known carcinogens and noncarcinogens. When applied to chemicals of unknown carcinogenicity, they must be used in a battery or a tiered testing protocol. This is necessary since false negatives may occur with any one assay. The size of the battery should depend upon the potential risk to humans. A chemical with high human exposure (e.g., food additives) should be tested with a larger number of assays than one with more limited human exposure. The tests should complement one another and detect different events. Thus bacterial or mammalian mutation, cell transformation, DNA repair and chromosome assays should all be included in a test battery. Results form in vitro assay can be utilized to determine whether the chemical should also be tested in an animal bioassay. While negative results in all tests may indicate low risk, a chemical with widespread human exposure may still be worth evaluating in a long-term animal bioassay. It is impossible to prove a chemical safe and without risk. Short-term assays do, however, provide a means for quickly obtaining reasonable information about the genotoxic potential of large numbers of chemicals.
R M Santella
Related Documents :
8921977 - Utility of a test for chromosomal malsegregation in saccharomyces cerevisiae strain d61...
23556847 - Design and fabrication of a bending rotation fatigue test rig for in situ electrochemic...
613937 - Short-term tests and mycotoxins.
1534777 - Risk assessment in immunotoxicology. i. sensitivity and predictability of immune tests.
15341177 - Geometric determinants to cement line debonding and osteonal lamellae failure in osteon...
22830297 - Successful desensitization with un-fractionated heparin in a patient with heparin aller...
Publication Detail:
Type:  In Vitro; Journal Article; Review    
Journal Detail:
Title:  Occupational medicine (Philadelphia, Pa.)     Volume:  2     ISSN:  0885-114X     ISO Abbreviation:  Occup Med     Publication Date:    1987 Jan-Mar
Date Detail:
Created Date:  1987-10-08     Completed Date:  1987-10-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605629     Medline TA:  Occup Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  39-46     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Assay
Carcinogens, Environmental / toxicity*
Mutagenicity Tests / methods
Mutagens / toxicity*
Reg. No./Substance:
0/Carcinogens, Environmental; 0/Mutagens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Scoliosis induced by medullary damage: an experimental study in rabbits.
Next Document:  Allergy to laboratory animals in health care personnel.