Document Detail

In vitro tenocyte metabolism in aging and oestrogen deficiency.
MedLine Citation:
PMID:  23274854     Owner:  NLM     Status:  Publisher    
Little is known about tendons and tenocyte biological behaviour during aging and, especially, oestrogen deficiency. The aim of this study was to evaluate in vitro the proliferation and metabolism of tenocytes isolated from the Achilles tendons of ovariectomised (OVX), middle-aged (OLD) and young (YOUNG) rats. An in vitro model of micro-wound healing was also used to assess age and oestrogen deficiency differences in tendon healing. In standard culture condition, OLD and OVX tenocytes showed a significantly lower proliferation rate, collagen I, aggrecan and elastin than YOUNG ones. In OVX group, fibronectin and elastin significantly decreased in comparison to YOUNG and OLD groups, respectively, whereas vascular endothelial growth factor and metalloproteinases-13 increased than those of both YOUNG and OLD groups. In the micro-wound healing model, tenocytes from both OVX and OLD showed a significantly lower healing rate, proliferation rate, collagen I and nitrix oxide in comparison to YOUNG. OVX elastin value was significantly lower than YOUNG one and OVX healing rate and cell migration speed, proliferation rate and fibronectin results were lower, whereas collagen III and metalloproteinase-13 higher in comparison to both YOUNG and OLD groups. These results highlighted how aging and, more significantly, oestrogen deficiency negatively affect tendon metabolism and healing. Our work improves the body of knowledge on the effects of senescence and oestrogen deficiency on tenocyte behaviour and allows further studies to find solution for the prevention of tendon injuries in aging and menopause.
P Torricelli; F Veronesi; S Pagani; N Maffulli; S Masiero; A Frizziero; M Fini
Related Documents :
3355354 - Balance function in elderly people who have and who have not fallen.
8859084 - Prevalence and associations of retinal vein occlusion in australia. the blue mountains ...
10808104 - Aging effects upon smooth pursuit induced by step-ramp stimulation.
16678754 - Correlation of grating acuity with letter recognition acuity in children with albinism.
10567004 - Genetic variability in plasma melatonin in sheep is due to pineal weight, not to variat...
10941744 - Evaluation of host preferences by helminths and ectoparasites among black-tailed jackra...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-29
Journal Detail:
Title:  Age (Dordrecht, Netherlands)     Volume:  -     ISSN:  1574-4647     ISO Abbreviation:  Age (Dordr)     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101250497     Medline TA:  Age (Dordr)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Preclinical and Surgical Studies Laboratory, Codivilla Putti Research Institute, Rizzoli Orthopaedic Institute, via di Barbiano, 1/10, 40136, Bologna, Italy,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Age-related neural correlates of cognitive task performance under increased postural load.
Next Document:  CTGF induces monocyte chemoattractant protein-1 expression to enhance monocyte migration in human sy...