| In vitro systems for exposure of lung cells to NO2 and O3. | |
| | |
MedLine Citation:
|
PMID: 6376819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In vitro studies of the effects of NO2 and O3 require development of methods for separation and culture of those lung cells that experience in vivo exposure, and also the design and construction of systems for controlled exposure of the cells to known concentrations of the gases. Separation of lung cell types has been accomplished by enzymatic dispersal of lung tissue and centrifugation of the mixed cells on media of various densities in order to separate the cells on the basis of buoyant density or sedimentation rate. The application of centrifugal elutriation has enabled separation of type II alveolar cells and Clara cells with a high degree of purity. Alveolar macrophages and endothelial cells have also been obtained in good yield. Exposure of cultured cells to test atmospheres requires precise control of pollutant levels, close contact of cells and gas without an intervening layer of medium, capability for prolonged exposure, and maintenance of sterile conditions, so that recovered cells can be cultured further or studied for other biological activity. Systems which meet these criteria include roller bottle cultures, petri dish cultures on rocker platforms, cell cultures on cellulose filters fed by perfusion of medium from the side opposite the cells, and cells grown in dishes with gas-permeable film bottoms. Systems that rely on solution of the gases in the overlaying medium do not resemble exposure conditions in vivo, and may not be suitable for studying effects of the poorly soluble oxidant gases. The cell exposure systems have not been used extensively for studies of the effects of pollutants on freshly isolated specific lung cell types. Such studies should be encouraged. |
| | |
Authors:
|
R E Rasmussen |
Related Documents
:
|
6109699 - Two rat mononuclear cell-derived slow reacting substances: kinetic evidence that the pe... 11704609 - Chromosome 16 loss of heterozygosity in tuberous sclerosis and sporadic lymphangiomyoma... 11237429 - Establishment and characterization of a human b cell line from the lung tissue of a pat... 19329539 - Characterization of mouse alveolar epithelial cell monolayers. 3011249 - Metabolic activation and cytotoxicity of 4-ipomeanol in human non-small cell lung cance... 3188809 - Effects of heparin treatments in vivo and in vitro on adrenal angiotensin ii receptors ... 3510929 - Direct identification of electrophysiologically monitored cells within intact mouse isl... 15037549 - Loss of cell polarity causes severe brain dysplasia in lgl1 knockout mice. 21538079 - [2,4-(13)c]β-hydroxybutyrate metabolism in astrocytes and c6 glioblastoma cells. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
|
Title: Journal of toxicology and environmental health Volume: 13 ISSN: 0098-4108 ISO Abbreviation: J Toxicol Environ Health Publication Date: 1984 |
Date Detail:
|
Created Date: 1984-08-20 Completed Date: 1984-08-20 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7513622 Medline TA: J Toxicol Environ Health Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 397-411 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cells, Cultured Humans Lung / drug effects* Macrophages / drug effects Methods Models, Biological Nitrogen Dioxide / toxicity* Ozone / toxicity* |
| Grant Support | |
ID/Acronym/Agency:
|
ES-01835/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
10028-15-6/Ozone; 10102-44-0/Nitrogen Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Early metabolic changes in response to lung injury: extrapolation from animals to humans.
Next Document: Oxidant-induced enhanced sensitivity to infection in animal models and their extrapolations to man.