| In vitro synthesis of mycolic acids by the fluffy layer fraction of Bacterionema matruchotii. | |
| | |
MedLine Citation:
|
PMID: 6703699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Biosynthetic activity for mycolic acid occurred in the fluffy layer fraction but not in the 5000g supernatant of Bacterionema matruchotii. With [1-14C]palmitic acid as precursor for the in vitro system, the predominant product was identified as C32:0 mycolic acid by radio-gas-liquid chromatographic (radio-GLC) and gas chromatographic/mass spectroscopic analyses; if [1-14C]stearic acid was used, two major radioactive peaks appeared on GLC: one corresponding to the peak of (C34:0 + C34:1) mycolic acids and the other to (C36:0 + C36:1) mycolic acids. By pyrolysis/radio-GLC analysis, C32:0 mycolic acid synthesized by [1-14C]palmitic acid was pyrolyzed at 300 degrees C to form palmitaldehyde (the mero moiety) and methyl palmitate (the branch moiety). The pH optimum for the incorporation of [1-14C]palmitate into bacterionema mycolic acids was 6.4 and the reaction required a divalent cation. The in vitro system utilized myristic, palmitic, stearic and oleic acids (probably via their activated forms) well as precursors, among which myristic and palmitic acids were more effective than the rest. Avidin showed no effect on the biosynthesis of mycolic acid from 14C-palmitate whereas cerulenin, a specific inhibitor of beta-ketoacyl synthetase in de novo fatty acid synthesis, inhibited the reaction at a relatively higher concentration. Thin-layer chromatographic analysis of lipids extracted from the reacting mixture without alkaline hydrolysis showed that both exogenous [1-14C]fatty acid and synthesized mycolic acids were bound to an unknown compound by an alkali-labile linkage and this association seemed to occur prior to the condensation of two molecules of fatty acid. |
| | |
Authors:
|
T Shimakata; M Iwaki; T Kusaka |
Related Documents
:
|
22754309 - A glutamic acid-producing lactic acid bacteria isolated from malaysian fermented foods. 573749 - Detection of mycolic acid in leprous tissue. 17805629 - Isolation and characterization of glycerides in human hair lipids by thin-layer and gas... 15558349 - Antifungal and sprout regulatory bioactivities of phenylacetic acid, indole-3-acetic ac... 8111249 - Incorporation of radioactive phosphate from adenosine triphosphate into phosphatidylino... 9461299 - Structure of a hydroxymycolic acid potentially involved in the synthesis of oxygenated ... 8831969 - Erythromycin biosynthesis: exploiting the catalytic versatility of the modular polyketi... 877849 - The gastric bladder: a continent reservoir for urinary diversion. 11087369 - Oxidative regulation of fatty acid-induced tau polymerization. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Archives of biochemistry and biophysics Volume: 229 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1984 Feb |
Date Detail:
|
Created Date: 1984-04-02 Completed Date: 1984-04-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 329-39 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Actinomycetaceae
/
analysis,
metabolism* Cations, Divalent Cell Fractionation* Chromatography, Gas Fatty Acids / metabolism Gas Chromatography-Mass Spectrometry Hydrogen-Ion Concentration Mycolic Acids / isolation & purification, metabolism* Palmitic Acid Palmitic Acids / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Cations, Divalent; 0/Fatty Acids; 0/Mycolic Acids; 0/Palmitic Acids; 57-10-3/Palmitic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protoporphyrinogen oxidation, an enzymatic step in heme and chlorophyll synthesis: partial character...
Next Document: Affinity labeling of spinach ferredoxin-NADP+ oxidoreductase with periodate-oxidized NADP+.