| In vitro susceptibility of Pythium insidiosum and a Lagenidium sp to itraconazole, posaconazole, voriconazole, terbinafine, caspofungin, and mefenoxam. | |
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MedLine Citation:
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PMID: 18980428 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate the radial growth assay for use in in vitro susceptibility testing of Pythium insidiosum and a Lagenidium sp and to assess susceptibility of representative isolates to itraconazole, posaconazole, voriconazole, terbinafine, caspofungin, and mefenoxam. SAMPLE POPULATION: 6 isolates each of P insidiosum and Lagenidium sp. PROCEDURES: Isolates were plated in triplicate onto agar supplemented with antifungal compounds at concentrations of 0.025 to 8 microg/mL. Isolates on dimethyl sulfoxide- and water-supplemented agar served as control samples. Effect of antifungal concentration on colony diameter was assessed with a mixed linear model. Assay variability was assessed with the coefficient of variation. RESULTS: Colony growth was uniform (mean intra-assay and interassay coefficients of variation were < 5%). Minimal inhibition was evident with voriconazole and posaconazole at 8 microg/mL. Terbinafine at 8 microg/mL significantly reduced growth of P insidiosum and at > or = 1 microg/mL significantly reduced growth of the Lagenidium sp. Caspofungin and mefenoxam (concentrations > or = 1 microg/mL and > or = 0.025 microg/mL, respectively) significantly reduced growth of both pathogens. Mefenoxam at 0.1 microg/mL caused > 50% growth inhibition in 11 of 12 isolates and at 1 microg/mL caused > 90% inhibition in all isolates. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that the radial growth assay was a simple, reproducible technique for susceptibility testing of P insidiosum and a Lagenidium sp. Azoles had limited activity, whereas terbinafine and caspofungin caused significant but minimal to moderate inhibition. Only mefenoxam had a profound effect on both pathogens at concentrations likely to be achievable in tissues. |
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Authors:
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Tina A Brown; Amy M Grooters; Giselle L Hosgood |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of veterinary research Volume: 69 ISSN: 0002-9645 ISO Abbreviation: Am. J. Vet. Res. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-04 Completed Date: 2008-12-16 Revised Date: 2009-03-31 |
Medline Journal Info:
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Nlm Unique ID: 0375011 Medline TA: Am J Vet Res Country: United States |
Other Details:
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Languages: eng Pagination: 1463-8 Citation Subset: IM |
Affiliation:
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Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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analogs & derivatives,
pharmacology Anti-Infective Agents / pharmacology* Echinocandins / pharmacology Itraconazole / pharmacology Lagenidium / drug effects*, growth & development Microbial Sensitivity Tests Naphthalenes / pharmacology Pyrimidines / pharmacology Pythium / drug effects*, growth & development Triazoles / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents; 0/Echinocandins; 0/Naphthalenes; 0/Pyrimidines; 0/Triazoles; 0/caspofungin; 0/mefenoxam; 0/posaconazole; 0/voriconazole; 56-41-7/Alanine; 84625-61-6/Itraconazole; 91161-71-6/terbinafine |
| Comments/Corrections | |
Comment In:
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Am J Vet Res. 2009 Feb;70(2):163; author reply 163
[PMID:
19231944
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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