Document Detail

In vitro susceptibilities of clinical yeast isolates to three antifungal agents determined by the microdilution method.
MedLine Citation:
PMID:  7666925     Owner:  NLM     Status:  MEDLINE    
A comparative evaluation of the in vitro susceptibilities of 597 clinical yeast isolates to amphotericin B, fluconazole, and 5-fluorocytosine (5FC) was conducted. The broth macrodilution reference method of the National Committee for Clinical Laboratory Standards (NCCLS, M27-P) was adapted to the microdilution method. Microdilution endpoints for amphotericin B were scored as the lowest concentration in which a score of 0 (complete absence of growth) was observed and for 5FC and fluconazole as the lowest concentration in which a score of 2 (prominent decrease in turbidity; MIC-2) was observed compared to the growth control. The MIC values were read after 24 and 48 h incubation. A broad range of MIC values was observed with each antifungal agent. Amphotericin B was very active (MIC90 < or = 1.0 microgram/ml) against all of the yeast isolates with the exception of C. lusitaniae (MIC90 > or = 2.0 micrograms/ml). Fluconazole was most active against C. parapsilosis (MIC90 of 1.0 microgram/ml) and least active against C. krusei (MIC90 of 32 micrograms/ml). 5FC was most active against C. albicans, C. parapsilosis, C. tropicalis, and T. glabrata (MIC90 < or = 1.0 microgram/ml) and was least active against C. krusei and C. lusitaniae (MIC90 > or = 16 micrograms/ml). These data indicate that the microdilution method, performed in accordance with M27-P, provides a means of testing larger numbers of yeast isolates against an array of antifungal agents and allows this to be accomplished in a reproducible and standardized manner. Given these results, it appears that the microdilution method may be a useful alternative to the macrodilution reference method for susceptibility testing of yeasts.
M A Pfaller; A L Barry
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Mycopathologia     Volume:  130     ISSN:  0301-486X     ISO Abbreviation:  Mycopathologia     Publication Date:  1995  
Date Detail:
Created Date:  1995-10-12     Completed Date:  1995-10-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505689     Medline TA:  Mycopathologia     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  3-9     Citation Subset:  IM    
Department of Pathology, University of Iowa College of Medicine, Iowa City, USA.
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MeSH Terms
Amphotericin B / pharmacology
Antifungal Agents / pharmacology*
Candida / drug effects
Dose-Response Relationship, Drug
Fluconazole / pharmacology
Flucytosine / pharmacology*
Microbial Sensitivity Tests* / standards
Mycoses / microbiology*
Yeasts / drug effects*
Reg. No./Substance:
0/Antifungal Agents; 1397-89-3/Amphotericin B; 2022-85-7/Flucytosine; 86386-73-4/Fluconazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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