| In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells. | |
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MedLine Citation:
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PMID: 17552898 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 microM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment. |
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Authors:
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H Kolárová; R Bajgar; K Tománková; E Krestýn; L Dolezal; J Hálek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-31 |
Journal Detail:
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Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: 56 Suppl 1 ISSN: 0862-8408 ISO Abbreviation: Physiol Res Publication Date: 2007 |
Date Detail:
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Created Date: 2008-01-16 Completed Date: 2008-04-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: Czech Republic |
Other Details:
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Languages: eng Pagination: S27-32 Citation Subset: IM |
Affiliation:
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Department of Medical Biophysics, Faculty of Medicine, Palacký University, Olomouc, Czech Republic. kol@tunw.upol.cz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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metabolism,
pathology,
therapy* Breast Neoplasms / metabolism, pathology, therapy* Cell Line, Tumor Cell Shape / drug effects Dose-Response Relationship, Drug Female Humans Organometallic Compounds / pharmacology, therapeutic use* Photochemotherapy* Photosensitizing Agents / pharmacology, therapeutic use* Reactive Oxygen Species / metabolism* Time Factors Ultrasonic Therapy* |
| Chemical | |
Reg. No./Substance:
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0/Organometallic Compounds; 0/Photosensitizing Agents; 0/Reactive Oxygen Species; 0/chloroaluminum disulfonated phthalocyanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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