Document Detail


In vitro study of the modification by bleomycin of thymidine phosphorylation activity in lectin-stimulated normal human lymphocytes.
MedLine Citation:
PMID:  2465077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated the effect of bleomycin (BLM) on thymidine phosphorylation in lectin-stimulated normal human lymphocytes. BLM reduces thymidine phosphorylation by decreasing the activity of thymidine kinase (TK). Accordingly, polyacrylamide gel electrophoresis (PAGE) of extracts of cells incubated for 48, 72 and 96 h showed here that this activity dropped 48, 65 and 67% respectively. The electrophoretic profiles of TK activity were similar but different in amplitude. These effects of the BLM were confirmed firstly by direct measurement of TK activity, secondly by amount of 3H-thymidine incorporation in the cultures before cell lysis. Both the measurement of TK activity and 3H-thymidine incorporation were correlated.
Authors:
A Sharif; Y Goussault
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer biochemistry biophysics     Volume:  10     ISSN:  0305-7232     ISO Abbreviation:  Cancer Biochem. Biophys.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1989-04-05     Completed Date:  1989-04-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7506524     Medline TA:  Cancer Biochem Biophys     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  17-24     Citation Subset:  IM    
Affiliation:
INSERM U-180 Faculté de Médicine, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Bleomycin / pharmacology*
Cells, Cultured
DNA Replication / drug effects
Humans
Kinetics
Lymphocyte Activation*
Lymphocytes / drug effects,  immunology*,  metabolism
Phosphorylation
Thymidine / metabolism*
Thymidine Kinase / metabolism*
Chemical
Reg. No./Substance:
11056-06-7/Bleomycin; 50-89-5/Thymidine; EC 2.7.1.21/Thymidine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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