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In vitro study on lethal effect of human choroidal melanoma OCM-1 cell line by repeating -70 degrees C freeze thawing.
MedLine Citation:
PMID:  17444038     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the effects of repeating -70 degrees C freeze thawing on human choroidal melanoma cell line OCM-1.
METHODS: OCM-1 cells were frozen by repeating -70 degrees C freeze thawing with various durations and frequencies. Then the inhibit rate of cells was examined by MTT essay. The cell viability was measured by monoclonal formation assay. We also used the HE staining, immunohistochemistry staining and the laser-scanning confocal microscopy (LSCM) to investigate the morphological changes of the cells.
RESULTS: The growth of OCM-1 cells was inhibited by repeating -70 degreesC freeze thawing in time-dependent and frequency-dependent manners (P < 0.01). Different morphous including necrosis and apoptosis of the cells could be observed after -70 degrees C freeze thawing by the LSCM.
CONCLUSION: Repeating -70 degrees C freeze thawing can not only kill cells directly and induce considerable cells to apoptosis, but also inhibit the growth of the survivals. The kill and wound ratio of the cells disposal with different times and frequencies present variance. And the distinction when treated with different frequencies during the same time is much more significant than different times with the same frequency, which guide clinical workers to choose repeating cryotherapy with short term method instead of single cryotherapy with long term in choroidal melanoma treatment. Eye Science
Authors:
Bin Liu; Yongping Li; Bo Zhang; Wenxin Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu     Volume:  23     ISSN:  1000-4432     ISO Abbreviation:  Yan Ke Xue Bao     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-04-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605666     Medline TA:  Yan Ke Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  31-6     Citation Subset:  IM    
Affiliation:
National Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University Guangzhou 510060, China.
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