| In vitro study of blood-contacting properties and effect on bacterial adhesion of a polymeric surface with immobilized heparin and sulphated hyaluronic acid. | |
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MedLine Citation:
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PMID: 11211093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The blood-contacting properties and the effect on bacterial adhesion of a material based on polyurethane and poly(amido-amine) (PUPA), both in its native form and with the anticoagulant molecules heparin or sulphated hyaluronic acid (HyalS3.5) electrostatically bonded to its surface, were evaluated and compared in vitro. The presence of the biological molecules on the surface was revealed by a dye test and ATR/FTIR analysis. Bound heparin was found to maintain its physiological action, in terms of thrombin inactivation, as well as did free heparin. Moreover, it reduced the degree of platelet adhesion. On the contrary, bound HyalS3.5 lost its anticoagulant activity, though it reduced platelet adhesion. The number of platelets on both modified surfaces was low. Their shape distribution, as determined by SEM, did not differ significantly on the two modified surfaces or with respect to the bare PUPA surface. HyalS3.5 and heparin also inhibited adhesion of Staphylococcus epidermidis to the material. A possible relationship between the platelet and bacterial adhesion is ascribed to the mediating role of plasma proteins. |
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Authors:
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A Magnani; R Barbucci; L Montanaro; C R Arciola; S Lamponi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomaterials science. Polymer edition Volume: 11 ISSN: 0920-5063 ISO Abbreviation: J Biomater Sci Polym Ed Publication Date: 2000 |
Date Detail:
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Created Date: 2001-02-08 Completed Date: 2001-06-07 Revised Date: 2008-02-20 |
Medline Journal Info:
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Nlm Unique ID: 9007393 Medline TA: J Biomater Sci Polym Ed Country: Netherlands |
Other Details:
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Languages: eng Pagination: 801-15 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biosystem Sciences and Technologies, University of Siena, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anticoagulants
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pharmacology Bacterial Adhesion Blood Platelets / cytology Cell Adhesion Fibrinogen / metabolism Heparin / pharmacology* Hyaluronic Acid / metabolism* Hydrogen-Ion Concentration Microscopy, Electron, Scanning Polyamines / pharmacology Polymers / metabolism* Polyurethanes / pharmacology Protein Binding Spectroscopy, Fourier Transform Infrared Staphylococcus / metabolism Thrombin / metabolism Thrombin Time Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Polyamines; 0/Polymers; 0/Polyurethanes; 123098-01-1/N2LL poly(amido-amine)-polyurethane copolymer; 9001-32-5/Fibrinogen; 9004-61-9/Hyaluronic Acid; 9005-49-6/Heparin; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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